L-012

Name: L-012
Brand: MedChemExpress
SKU: HY-118315
Rating: 5.0 (2 reviews)

Cat. No.: HY-118315 Purity: 99.63%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

L-012 is a luminal-based chemiluminescent probe. L-012 sodium salt can detect NADPH oxidase (Nox)-derived superoxide and nitrogen species (reactive oxygen species (ROS) and reactive nitrogen species (RNS)).

For research use only. We do not sell to patients.

CAS No. : 143323-55-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of L-012:

L 012 sodium salt In-stock

2 Publications Citing Use of MCE L-012

Histological Imaging/Staining

Bio/Physico-chemical Assay

: L-012 purchased from MedChemExpress. Usage Cited in: Cell Calcium. 2026 Mar:134:103122. [Abstract]; Activity of NOX2 in cells treated with 100ng/ml PMA, PMA+ 30 μM GSK2795039and 10 μM S-023–0515 was measured by L-012 (10 μM, 10 min at 37 °C) luminescence kinetics over 90 min.

: L-012 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2024 Jul 22:28:101163. [Abstract]; Live imaging of small animals and quantitative analysis of ROS stained by L012 in periodontal tissue with different treatments (7d).

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References
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Description

In Vitro

1. Solution preparation^[1]
1.1 Preparation of stock solution
Solvent: DMSO or ultrapure water
Concentration: 10 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2 Assay for ROS Generated by Cell-Free Systems.
2.1 In the presence of 1 mM hypoxanthine in KRP (Krebs-Ringer phosphate buffer), superoxide is generated by xanthine oxidase (6.4 mU/mlL) at 37°C.
2.2 Concentrations of L-012, luminol and MCLA used for the assay are 4 μM, 100 μM, and 4 μM, respectively.
2.3 The hydroxyl radical is generated in KRP by incubating 100 μM DTPA, 1 mM H2O2, and 50 μM FeSO4 at 37°C.
2.4 Detection using in Luminescence Reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NF-κB-luciferase reporter mice^[2].
Luciferase activity was monitored in vivo by injecting the substrate luciferin.
L-012 (25 mg/kg in 100 μL) is administered intravenously (iv), intraperitoneally (ip), or subcutaneously (sc).
Detection using in vivo imaging.
Image exposure times are between 5 s and 2 min, depending on the signal strength.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

288.69

Formula

C₁₃H₉ClN₄O₂

CAS No.

143323-55-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C2C(Cl)=NC(C3=CC=CC=C3)=C1N)NNC2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (346.39 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4639 mL	17.3196 mL	34.6392 mL
5 mM	0.6928 mL	3.4639 mL	6.9278 mL
10 mM	0.3464 mL	1.7320 mL	3.4639 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (253 KB) HNMR (165 KB) RP-HPLC (169 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Imada I, et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012[J]. Analytical biochemistry, 1999, 271(1): 53-58. [Content Brief]

[2]. Kielland A, et al. In vivo imaging of reactive oxygen and nitrogen species in inflammation using the luminescent probe L-012. Free Radic Biol Med. 2009;47(6):760-766. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4639 mL	17.3196 mL	34.6392 mL	86.5981 mL
	5 mM	0.6928 mL	3.4639 mL	6.9278 mL	17.3196 mL
	10 mM	0.3464 mL	1.7320 mL	3.4639 mL	8.6598 mL
	15 mM	0.2309 mL	1.1546 mL	2.3093 mL	5.7732 mL
	20 mM	0.1732 mL	0.8660 mL	1.7320 mL	4.3299 mL
	25 mM	0.1386 mL	0.6928 mL	1.3856 mL	3.4639 mL
	30 mM	0.1155 mL	0.5773 mL	1.1546 mL	2.8866 mL
	40 mM	0.0866 mL	0.4330 mL	0.8660 mL	2.1650 mL
	50 mM	0.0693 mL	0.3464 mL	0.6928 mL	1.7320 mL
	60 mM	0.0577 mL	0.2887 mL	0.5773 mL	1.4433 mL
	80 mM	0.0433 mL	0.2165 mL	0.4330 mL	1.0825 mL
	100 mM	0.0346 mL	0.1732 mL	0.3464 mL	0.8660 mL

L-012 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-012143323-55-1L012L 012Fluorescent DyeProbesNeutrophilsFree RadicalsDisease PathogenesisChemiluminescenceInhibitorinhibitorinhibit

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L-012

Customer Review

Other Forms of L-012:

L-012 Related Antibodies

2 Publications Citing Use of MCE L-012

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Molarity Calculator

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Complete Stock Solution Preparation Table

L-012 Related Classifications

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