1. Academic Validation
  2. [Effect of Leonurus stachydrine on myocardial cell hypertrophy]

[Effect of Leonurus stachydrine on myocardial cell hypertrophy]

  • Zhong Yao Cai. 2012 Jun;35(6):940-3.
Wei Guo 1 Chen Zhang Yue-Ling Liao Rong Lv Hong-Chang Wei
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Affiliation

PMID: 23236831
Abstract

Objective: To investigate the effects of Leonurus stachydrine on myocardial cell hypertrophy induced by angiotensin II in rats.

Methods: Myocardial cells of neonatal rats were incubated with angiotensin II for 48 hours to establish myocardial cell hypertrophy models. The cell surface area and protein/DNA ratio were detected as index for hypertrophy of myocardial cells. ROS positive myocardial cell were counted by means of flow cytometry, the expression of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus were also detected by Western blot.

Results: Various concentrations of stachydrine intervention (10(-6) - 10(-4) mol/L) showed better anti-hypertrophic effect and decreased the number of ROS positive myocardial cell (P<0.05). Meanwhile, intervention of stachydrine significantly suppressed the level of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus (P<0.05).

Conclusion: Stachydrine can inhibit the NF-kappaB signal pathway, and this may be ralated to the mechanism of anti-hypertrophic.

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