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Stachydrine hydrochloride 

Cat. No.: HY-N0738 Purity: >98.0%
Handling Instructions

Stachydrine hydrochloride is the major active constituent of Herba Leonuri, which is a potential therapy for cardiovascular diseases. Stachydrine can inhibit the NF-κB signal pathway. Anti-hypertrophic activities.

For research use only. We do not sell to patients.

Stachydrine hydrochloride Chemical Structure

Stachydrine hydrochloride Chemical Structure

CAS No. : 4136-37-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

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Description

Stachydrine hydrochloride is the major active constituent of Herba Leonuri, which is a potential therapy for cardiovascular diseases[2]. Stachydrine can inhibit the NF-κB signal pathway. Anti-hypertrophic activities[1].

IC50 & Target

p65

 

In Vitro

Intervention of Stachydrine significantly suppresses the level of p-IκB (ser32) protein in the cytosol and NF-κB (p65) protein in the nucleus (P<0.05)[1].
Treatment with Stachydrine hydrochloride (50 µM, 200 µM, 500 µM and 1000 µM) noticeably inhibited MCF-7 and T47D cell proliferation in dose- and time-dependent manner[2].
High concentrations (500 µM and 1000 µM) of Stachydrine significantly increased the frequency of both studied cell lines at the G1 phase of cell cycle, suggesting that Stachydrine hydrochloride could cause cell cycle arrest[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

179.64

Formula

C₇H₁₄ClNO₂

CAS No.

4136-37-2

SMILES

OC([[email protected]](CCC1)[N+]1(C)C)=O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 120 mg/mL (668.00 mM; Need ultrasonic)

DMSO : 120 mg/mL (668.00 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.5667 mL 27.8334 mL 55.6669 mL
5 mM 1.1133 mL 5.5667 mL 11.1334 mL
10 mM 0.5567 mL 2.7833 mL 5.5667 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 3 mg/mL (16.70 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 3 mg/mL (16.70 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 3 mg/mL (16.70 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay

Cell Proliferation Assay[2]
Cell Cycle Analysis[2]
MCF-7 and T47D cells
50 µM, 200 µM, 500 µM and 1000 µM
24 and 48 hours
CCK-8 cell counting assay is used to detect proliferation of MCF-7 and T47D cells when treated with Stachydrine hydrochloride at four different concentrations (50 µM, 200 µM, 500 µM and 1000 µM) for 24 and 48 hours. Cell cycle analysis of MCF-7 and T47D cells is determined by flow cytometry. Experiments are performed in triplicates and representative images are presented.
Note: Inhibited proliferation and induced G1 phase arrest in MCF-7 and T47D cells.
[1] Guo W, et al. Effect of Leonurus Stachydrine on myocardial cell hypertrophy. Zhong Yao Cai. 2012 Jun;35(6):940-3. https://www.ncbi.nlm.nih.gov/pubmed/23236831 [2] Am J Transl Res. 2017 Apr 15;9(4):1834-1844. eCollection 2017. Stachydrine hydrochloride inhibits proliferation and induces apoptosis of breast cancer cells via inhibition of Akt and ERK pathways. Wang M1, Shu ZJ1, Wang Y1, Peng W1 https://www.ncbi.nlm.nih.gov/pubmed/28469788

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >98.0%

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Keywords:

StachydrineNF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Product Name:
Stachydrine hydrochloride
Cat. No.:
HY-N0738
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