Cat. No.: HY-N0738

Stachydrine hydrochloride

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Stachydrine hydrochloride is the major active constituent of Leonurus artemisia, which is a potential therapy for cardiovascular diseases. Stachydrine can inhibit the NF-κB signal pathway. Anti-hypertrophic activities.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 4136-37-2
Formula: C₇H₁₄ClNO₂
Molecular Weight:179.64

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Stachydrine hydrochloride Chemical Structure

CAS No.: 4136-37-2

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Stachydrine hydrochloride is the major active constituent of Leonurus artemisia, which is a potential therapy for cardiovascular diseases^[2]. Stachydrine can inhibit the NF-κB signal pathway. Anti-hypertrophic activities^[1].

IC₅₀ & Target^[1]

p65

All NF-κB Isoforms :

All Endogenous Metabolite Isoforms :

In Vitro

Intervention of Stachydrine significantly suppresses the level of p-IκB (ser32) protein in the cytosol and NF-κB (p65) protein in the nucleus (P<0.05)^[1].
Treatment with Stachydrine hydrochloride (50 μM, 200 μM, 500 μM and 1000 μM) noticeably inhibited MCF-7 and T47D cell proliferation in dose- and time-dependent manner^[2].
High concentrations (500 μM and 1000 μM) of Stachydrine significantly increased the frequency of both studied cell lines at the G1 phase of cell cycle, suggesting that Stachydrine hydrochloride could cause cell cycle arrest^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 4136-37-2
Appearance Solid
Molecular Weight 179.64
Formula C₇H₁₄ClNO₂
Color White to off-white
SMILES

C[N+]1(CCC[C@H]1C([O-])=O)C.Cl
Structure Classification
- Alkaloids
- Pyrrole Alkaloids
Initial Source
- Plants
- Labiatae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (9)

All Cell Proliferation/Viability AssayCell Imaging/StainingWB

Sort by:

Journal Impact Factor

Phytomedicine

Motherwort synergized with oxytocin for postpartum hemorrhage prevention: Integrated clinical efficacy and mechanism exploration. [Abstract]

2025 Sep:145:156951. PMID: 40527062
J Agric Food Chem

Stachydrine Mediates Rapid Vascular Relaxation: Activation of Endothelial Nitric Oxide Synthase Involving AMP-Activated Protein Kinase and Akt Phosphorylation in Vascular Endothelial Cells. [Abstract]

2019 Sep 4;67(35):9805-9811. PMID: 31407895
Food Funct

Stachydrine ameliorates the progression of intervertebral disc degeneration via the PI3K/Akt/NF-κB signaling pathway: in vitro and in vivo studies. [Abstract]

2020 Dec 1;11(12):10864-10875. PMID: 33245081
Mol Plant Microbe Interact

Stachydrine Catabolism Contributes to an Optimal Root Nodule Symbiosis Between Sinorhizobium meliloti and Medicago sativa. [Abstract]

2025 Aug 10. PMID: 40783937
Toxicol Appl Pharmacol

Proline betaine facilitates angiogenesis in bronchopulmonary dysplasia. [Abstract]

2025 May:498:117301. PMID: 40089188
J Food Meas Charact

2024 Jul 12.
Exp Eye Res

Stachydrine mitigates high glucose-induced apoptosis in human lens epithelial cells by activating mitophagy. [Abstract]

2025 Nov:260:110568. PMID: 40816531
Rev Bras Farmacogn

2025 Sep 4.
J Cardiothorac Surg

Stachydrine ameliorates hypoxia reoxygenation injury of cardiomyocyte via enhancing SIRT1-Nrf2 pathway. [Abstract]

2023 Sep 26;18(1):265. PMID: 37752609

Stachydrine hydrochloride purchased from MedChemExpress. Usage Cited in: J Cardiothorac Surg. 2023 Sep 26;18(1):265. [Abstract]

H9c2 cells were incubated with Stachydrine (STA) (0, 10, 20, 50, 100, 200 µM) for 24 h. Cell viability was evaluated by CCK-8 assay.

Stachydrine hydrochloride purchased from MedChemExpress. Usage Cited in: J Cardiothorac Surg. 2023 Sep 26;18(1):265. [Abstract]

STA inhibits apoptosis in cardiomyocytes with H/R. H9c2 cells were pretreated with 50 µM Stachydrine (STA) for 2 h and then subjected to 16 h H/R stained with TUNEL.

Stachydrine hydrochloride purchased from MedChemExpress. Usage Cited in: J Cardiothorac Surg. 2023 Sep 26;18(1):265. [Abstract]

H9c2 cells were pretreated with 50 µM Stachydrine (STA) for 2 h and then subjected to H/R for 16 h. Antioxidative protein levels were determined by western blot

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (556.67 mM; Need ultrasonic)

DMSO : 100 mg/mL (556.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.5667 mL

27.8334 mL

55.6669 mL

5 mM

1.1133 mL

5.5667 mL

11.1334 mL

10 mM

0.5567 mL

2.7833 mL

5.5667 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (6.96 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.25 mg/mL (6.96 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (6.96 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (556.67 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protocol

Cell Assay

Cell Proliferation Assay^[2]
Cell Cycle Analysis^[2]
MCF-7 and T47D cells
50 µM, 200 µM, 500 µM and 1000 µM
24 and 48 hours
CCK-8 cell counting assay is used to detect proliferation of MCF-7 and T47D cells when treated with Stachydrine hydrochloride at four different concentrations (50 µM, 200 µM, 500 µM and 1000 µM) for 24 and 48 hours. Cell cycle analysis of MCF-7 and T47D cells is determined by flow cytometry. Experiments are performed in triplicates and representative images are presented.
Note: Inhibited proliferation and induced G1 phase arrest in MCF-7 and T47D cells.
[1] Guo W, et al. Effect of Leonurus Stachydrine on myocardial cell hypertrophy. Zhong Yao Cai. 2012 Jun;35(6):940-3. https://www.ncbi.nlm.nih.gov/pubmed/23236831 [2] Am J Transl Res. 2017 Apr 15;9(4):1834-1844. eCollection 2017. Stachydrine hydrochloride inhibits proliferation and induces apoptosis of breast cancer cells via inhibition of Akt and ERK pathways. Wang M1, Shu ZJ1, Wang Y1, Peng W1 https://www.ncbi.nlm.nih.gov/pubmed/28469788

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Guo W, et al. Effect of Leonurus Stachydrine on myocardial cell hypertrophy. Zhong Yao Cai. 2012 Jun;35(6):940-3. [Content Brief]

[2]. Wang M, et al. Stachydrine hydrochloride inhibits proliferation and induces apoptosis of breast cancer cells via inhibition of Akt and ERK pathways. Am J Transl Res. 2017 Apr 15;9(4):1834-1844. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	5.5667 mL	27.8334 mL	55.6669 mL	139.1672 mL
	5 mM	1.1133 mL	5.5667 mL	11.1334 mL	27.8334 mL
	10 mM	0.5567 mL	2.7833 mL	5.5667 mL	13.9167 mL
	15 mM	0.3711 mL	1.8556 mL	3.7111 mL	9.2778 mL
	20 mM	0.2783 mL	1.3917 mL	2.7833 mL	6.9584 mL
	25 mM	0.2227 mL	1.1133 mL	2.2267 mL	5.5667 mL
	30 mM	0.1856 mL	0.9278 mL	1.8556 mL	4.6389 mL
	40 mM	0.1392 mL	0.6958 mL	1.3917 mL	3.4792 mL
	50 mM	0.1113 mL	0.5567 mL	1.1133 mL	2.7833 mL
	60 mM	0.0928 mL	0.4639 mL	0.9278 mL	2.3195 mL
	80 mM	0.0696 mL	0.3479 mL	0.6958 mL	1.7396 mL
	100 mM	0.0557 mL	0.2783 mL	0.5567 mL	1.3917 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Stachydrine hydrochloride

9 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Stachydrine hydrochloride

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Peptidomimetic Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Medicine Food Homology Compound Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

Alkaloids Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Food-Sourced Compound Library

Anti-Liver Cancer Compound Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Stachydrine hydrochloride

All NF-κB Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (9)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Stachydrine hydrochloride Related Classifications

HY-N0738 Related Classifications