2. NF-κB Metabolic Enzyme/Protease
  3. NF-κB Endogenous Metabolite
  4. Stachydrine

Stachydrine is a major constituent of Chinese herb leonurus heterophyllus sweet used to promote blood circulation and dispel blood stasis. Stachydrine can inhibit the NF-κB signal pathway.

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CAS No. : 471-87-4

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Based on 9 publication(s) in Google Scholar

Other Forms of Stachydrine:

Stachydrine Related Antibodies

Top Publications Citing Use of Products

    Stachydrine purchased from MedChemExpress. Usage Cited in: J Cardiothorac Surg. 2023 Sep 26;18(1):265.  [Abstract]

    H9c2 cells were incubated with Stachydrine (STA) (0, 10, 20, 50, 100, 200 µM) for 24 h. Cell viability was evaluated by CCK-8 assay.

    Stachydrine purchased from MedChemExpress. Usage Cited in: J Cardiothorac Surg. 2023 Sep 26;18(1):265.  [Abstract]

    STA inhibits apoptosis in cardiomyocytes with H/R. H9c2 cells were pretreated with 50 µM Stachydrine (STA) for 2 h and then subjected to 16 h H/R stained with TUNEL.

    Stachydrine purchased from MedChemExpress. Usage Cited in: J Cardiothorac Surg. 2023 Sep 26;18(1):265.  [Abstract]

    H9c2 cells were pretreated with 50 µM Stachydrine (STA) for 2 h and then subjected to H/R for 16 h. Antioxidative protein levels were determined by western blot

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Stachydrine is a major constituent of Chinese herb leonurus heterophyllus sweet used to promote blood circulation and dispel blood stasis. Stachydrine can inhibit the NF-κB signal pathway.

    IC50 & Target[1]

    p65

     

    In Vitro

    Stachydrine can inhibit the NF-κB signal pathway, and this may be related to the mechanism of anti-hypertrophic. Intervention of stachydrine significantly suppresses the level of p-IκB protein in the cytosol and NF-κB protein in the nucleus [1]. Tissue factor mRNA is decreased in stachydrine-treated human umbilical vein endothelial cells. Stachydrine attenuates the decline of human umbilical vein endothelial cells viability and the increase of LDH activity induced by anoxia-reoxygenation[2]. A dose dependent decrease in expression of mRNA, and protein levels are observed in stachydrine-treated human prostate cancer cells (PC-3 and LNcaP)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Stachydrine Related Antibodies
    In Vivo

    Stachydrine attenuates norepinephrine-induced cardiomyocyte hypertrophy and has potential protective effects against β-adrenergic receptor induced Ca2+ mishandling[4]. Stachydrine treatment reduces the expressions of PERK, CHOP, and caspase-3 in the endoplasmic reticulum stress-related apoptosis pathway[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    143.18

    Formula

    C7H13NO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[N+]1(C)[C@H](C([O-])=O)CCC1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (698.42 mM; Need ultrasonic)

    DMSO : 100 mg/mL (698.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.9842 mL 34.9211 mL 69.8422 mL
    5 mM 1.3968 mL 6.9842 mL 13.9684 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 110 mg/mL (768.26 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Dosing volume
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    SDS (396 KB)

    COA (253 KB) HNMR (150 KB) RP-HPLC (243 KB) MS (227 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [3]

    Cytotoxicity is determined by colorimetric MTT cleavage assay. Briefly, human umbilical vein endothelial cells (HUVECs) are plated in triplicate in 96-well culture plates, and treated with different final concentrations (0.01, 0.1, 1, 10, 100 μM) of stachydrine respectively for 24 hours. After incubation, culture media are discarded and new culture media containing 0.5mg/mL of MTT are added. The plates are further incubated at 37°C for 4 hours. After the incubation, culture media are discarded and 0.1 mL of dimethyl sulfoxide (DMSO) is added to each well to solubilize the formazine crystals. The absorbance (OD) is measured at 540 nm using a microplate reader[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [5]

    Rats: Ventricular myocytes from 1-day-old Wistar rats are isolated and cultured in DMEM/F12 with 1 μM norepinephrine in the presence or absence of 10 μM stachydrine for 72 h. Cardiomyocytes hypertrophy is evaluated by cell surface area, total protein/DNA content, β/α-MHC mRNA ratio. While calcium handling function is evaluated by Ca2+-transient amplitude and decay, SERCA2a activity and expression, PLN expression and phosphorylation. β1-adrenergic receptor system activation is evaluated by the content of cAMP and the activation of PKA[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 6.9842 mL 34.9211 mL 69.8422 mL 174.6054 mL
    5 mM 1.3968 mL 6.9842 mL 13.9684 mL 34.9211 mL
    10 mM 0.6984 mL 3.4921 mL 6.9842 mL 17.4605 mL
    15 mM 0.4656 mL 2.3281 mL 4.6561 mL 11.6404 mL
    20 mM 0.3492 mL 1.7461 mL 3.4921 mL 8.7303 mL
    25 mM 0.2794 mL 1.3968 mL 2.7937 mL 6.9842 mL
    30 mM 0.2328 mL 1.1640 mL 2.3281 mL 5.8202 mL
    40 mM 0.1746 mL 0.8730 mL 1.7461 mL 4.3651 mL
    50 mM 0.1397 mL 0.6984 mL 1.3968 mL 3.4921 mL
    60 mM 0.1164 mL 0.5820 mL 1.1640 mL 2.9101 mL
    80 mM 0.0873 mL 0.4365 mL 0.8730 mL 2.1826 mL
    100 mM 0.0698 mL 0.3492 mL 0.6984 mL 1.7461 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Stachydrine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Stachydrine471-87-4NF-κBEndogenous MetaboliteNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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