1. NF-κB
    Metabolic Enzyme/Protease
  2. NF-κB
    Endogenous Metabolite
  3. Stachydrine

Stachydrine 

Cat. No.: HY-N0298 Purity: >98.0%
Handling Instructions

Stachydrine is a major constituent of Chinese herb leonurus heterophyllus sweet used to promote blood circulation and dispel blood stasis. Stachydrine can inhibit the NF-κB signal pathway.

For research use only. We do not sell to patients.

Stachydrine Chemical Structure

Stachydrine Chemical Structure

CAS No. : 471-87-4

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10 mM * 1 mL in DMSO USD 66 In-stock
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10 mg USD 60 In-stock
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50 mg USD 120 In-stock
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100 mg USD 168 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Stachydrine is a major constituent of Chinese herb leonurus heterophyllus sweet used to promote blood circulation and dispel blood stasis. Stachydrine can inhibit the NF-κB signal pathway.

IC50 & Target[1]

p65

 

Human Endogenous Metabolite

 

In Vitro

Stachydrine can inhibit the NF-κB signal pathway, and this may be related to the mechanism of anti-hypertrophic. Intervention of stachydrine significantly suppresses the level of p-IκB protein in the cytosol and NF-κB protein in the nucleus [1]. Tissue factor mRNA is decreased in stachydrine-treated human umbilical vein endothelial cells. Stachydrine attenuates the decline of human umbilical vein endothelial cells viability and the increase of LDH activity induced by anoxia-reoxygenation[2]. A dose dependent decrease in expression of mRNA, and protein levels are observed in stachydrine-treated human prostate cancer cells (PC-3 and LNcaP)[3].

In Vivo

Stachydrine attenuates norepinephrine-induced cardiomyocyte hypertrophy and has potential protective effects against β-adrenergic receptor induced Ca2+ mishandling[4]. Stachydrine treatment reduces the expressions of PERK, CHOP, and caspase-3 in the endoplasmic reticulum stress-related apoptosis pathway[5].

Molecular Weight

143.18

Formula

C₇H₁₃NO₂

CAS No.

471-87-4

SMILES

C[N+]1(C)[[email protected]](C([O-])=O)CCC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (698.42 mM; Need ultrasonic)

DMSO : 100 mg/mL (698.42 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.9842 mL 34.9211 mL 69.8422 mL
5 mM 1.3968 mL 6.9842 mL 13.9684 mL
10 mM 0.6984 mL 3.4921 mL 6.9842 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[3]

Cytotoxicity is determined by colorimetric MTT cleavage assay. Briefly, human umbilical vein endothelial cells (HUVECs) are plated in triplicate in 96-well culture plates, and treated with different final concentrations (0.01, 0.1, 1, 10, 100 μM) of stachydrine respectively for 24 hours. After incubation, culture media are discarded and new culture media containing 0.5mg/mL of MTT are added. The plates are further incubated at 37°C for 4 hours. After the incubation, culture media are discarded and 0.1 mL of dimethyl sulfoxide (DMSO) is added to each well to solubilize the formazine crystals. The absorbance (OD) is measured at 540 nm using a microplate reader[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[5]

Rats: Ventricular myocytes from 1-day-old Wistar rats are isolated and cultured in DMEM/F12 with 1 μM norepinephrine in the presence or absence of 10 μM stachydrine for 72 h. Cardiomyocytes hypertrophy is evaluated by cell surface area, total protein/DNA content, β/α-MHC mRNA ratio. While calcium handling function is evaluated by Ca2+-transient amplitude and decay, SERCA2a activity and expression, PLN expression and phosphorylation. β1-adrenergic receptor system activation is evaluated by the content of cAMP and the activation of PKA[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >98.0%

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Keywords:

StachydrineNF-κBEndogenous MetaboliteNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Stachydrine
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