1. Academic Validation
  2. Cancer cell-derived lymphotoxin mediates reciprocal tumour-stromal interactions in human ovarian cancer by inducing CXCL11 in fibroblasts

Cancer cell-derived lymphotoxin mediates reciprocal tumour-stromal interactions in human ovarian cancer by inducing CXCL11 in fibroblasts

  • J Pathol. 2014 Jan;232(1):43-56. doi: 10.1002/path.4258.
Tat-San Lau 1 Tony Kwok-Hung Chung Tak-Hong Cheung Loucia Kit-Ying Chan Leonard Wing-Hong Cheung So-Fan Yim Nelson Shing-Shun Siu Kwok-Wai Lo May Mei-Yung Yu Hagen Kulbe Frances R Balkwill Joseph Kwong
Affiliations

Affiliation

  • 1 Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong, China.
Abstract

We have investigated the role of cytokine lymphotoxin in tumour-stromal interactions in human ovarian Cancer. We found that lymphotoxin overexpression is commonly shared by the Cancer cells of various ovarian Cancer subtypes, and lymphotoxin-beta receptor (LTBR) is expressed ubiquitously in both the Cancer cells and cancer-associated fibroblasts (CAFs). In monoculture, we showed that ovarian Cancer cells are not the major lymphotoxin-responsive cells. On the other hand, our co-culture studies demonstrated that the Cancer cell-derived lymphotoxin induces chemokine expression in stromal fibroblasts through LTBR-NF-κB signalling. Amongst the chemokines being produced, we found that fibroblast-secreted CXCL11 promotes proliferation and migration of ovarian Cancer cells via the Chemokine Receptor CXCR3. CXCL11 is highly expressed in CAFs in ovarian Cancer biopsies, while CXCR3 is found in malignant cells in primary ovarian tumours. Additionally, the overexpression of CXCR3 is significantly associated with the tumour grade and lymph node metastasis of ovarian Cancer, further supporting the role of CXCR3, which interacts with CXCL11, in promoting growth and metastasis of human ovarian Cancer. Taken together, these results demonstrated that cancer-cell-derived lymphotoxin mediates reciprocal tumour-stromal interactions in human ovarian Cancer by inducing CXCL11 in fibroblasts. Our findings suggest that lymphotoxin-LTBR and CXCL11-CXCR3 signalling represent therapeutic targets in ovarian Cancer.

Keywords

CXCL11; CXCR3; Lymphotoxin; cancer-associated fibroblasts; ovarian cancer; tumour microenvironment; tumour-stromal interactions.

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