2. Academic Validation
  3. Anti-inflammatory activity of compounds from Boesenbergia longiflora rhizomes

Anti-inflammatory activity of compounds from Boesenbergia longiflora rhizomes

  • J Ethnopharmacol. 2014 Jun 11;154(2):453-61. doi: 10.1016/j.jep.2014.04.034.
Teeratad Sudsai 1,  Samran Prabpai 2,  Palangpon Kongsaeree 2,  Chatchai Wattanapiromsakul 3,  Supinya Tewtrakul 4
Affiliations

Affiliations

  • 1 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand; Faculty of Oriental Medicine, Rangsit University, Patumthani 12000, Thailand.
  • 2 Department of Chemistry and Center for Excellence in Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
  • 3 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112, Thailand.
  • 4 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand. Electronic address: [email protected].
PMID: 24786574 DOI: 10.1016/j.jep.2014.04.034
Abstract

Ethnopharmacological relevance: The rhizomes of Boesenbergia longiflora (Wall.) Kuntze have been traditionally used in treatment of inflammatory bowel disease, ulcerative colitis, aphthous ulcer and abscess. Our previous study indicated that CHCl3 fractions of Boesenbergia longiflora had potential on anti-inflammatory properties. In the present study, we investigated the active constituents of this plant for anti-inflammatory activity in order to support its traditional use.

Material and methods: The CHCl3 fraction was isolated using chromatographic techniques. Isolated compounds were tested using relevant in vitro anti-inflammatory assays against LPS-induced NO and TNF-α releases as well as their mechanisms in transcription levels in murine macrophage RAW264.7 cells.

Results: The isolation of the CHCl3 fraction from Boesenbergia longiflora rhizomes led to the isolation of three new daucane Sesquiterpenes, which were identified as 8-hydroxy-dauca-9, 11-diene-7-one (longiferone A; 1), dauca-8, 11-diene-7-one (longiferone B; 2) and dauca-8, 11-diene-7, 10-dione (longiferone C; 3); together with four known Flavonoids, six known diarylheptanoids as well as one sterol. The longiferone B (2) and longiferone C (3) showed anti-inflammatory activity against NO release with IC50 values of 21.0 and 31.3µM, respectively. Longiferone B (2) also suppressed the iNOS and COX-2 mRNA expression. Moreover, the Flavonoids and diarylheptanoids inhibited NO and TNF-α production in a dose dependent manner.

Conclusion: This study demonstrated that Sesquiterpenes, diarylheptanoids and some methoxyflavonoids found in Boesenbergia longiflora are responsible for anti-inflammatory activity.

Keywords

Anti-inflammatory activity; Boesenbergia longiflora; Daucane sesquiterpenes; Diarylheptanoids; Flavonoids.

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