2. Academic Validation
  3. Identification of terpenoids from Rubus corchorifolius L. f. leaves and their anti-proliferative effects on human cancer cells

Identification of terpenoids from Rubus corchorifolius L. f. leaves and their anti-proliferative effects on human cancer cells

  • Food Funct. 2017 Mar 22;8(3):1052-1060. doi: 10.1039/c6fo01343k.
Xuexiang Chen 1,  Zili Gao 2,  Mingyue Song 2,  Wen Ouyang 3,  Xian Wu 2,  Yunjiao Chen 4,  Liping Zhou 4,  Dixon William 2,  Xiaokun Cai 2,  Yong Cao 4,  Shuangde Zhou 5,  Zhonghai Tang 5,  Hang Xiao 6
Affiliations

Affiliations

  • 1 College of Public Health, Guangzhou Medical University, Guangzhou 511436, P. R. China and College of Food Science, South China Agricultural University, Guangzhou 510642, P. R. China. [email protected] and Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA. [email protected].
  • 2 Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA. [email protected].
  • 3 Pharmacy College, Hunan University of Traditional Chinese Medicine, Changsha 410007, P. R. China.
  • 4 College of Food Science, South China Agricultural University, Guangzhou 510642, P. R. China. [email protected].
  • 5 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, P. R. China.
  • 6 Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA. [email protected] and College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, P. R. China.
PMID: 28134947 DOI: 10.1039/c6fo01343k
Abstract

The leaves of Rubus corchorifolius L. f. have been consumed as a herbal tea for a long time. In this study, two novel (1 and 5) and four known (2, 3, 4 and 6) Terpenoids were isolated from the leaves of Rubus corchorifolius L. f. Structural analysis was performed using various spectroscopic methods (1H NMR, 13C NMR and MS) to identify the following six compounds: (16α)-16,17,18-trihydroxy-ent-kauran-18-O-β-d-glucoside (1), ent-16β,17-dialkyl-3-oxygen-kaurane (2), ent-kaurane-3α,16β,17-triol (3), ent-kaurane(5R,8S,9R,10R,13R,16R)-2-one-16α,17-diol (4), (16R)-16β,17,19-trihydroxy-ent-kaur-3-one (5) and ent-16α,17-dihydroxy-kauran-19-oic-acid (6). These compounds showed different inhibitory effects on various human Cancer cells. Compounds 3 and 6 exhibited stronger inhibitory effects on human colon Cancer HCT116 cells than the other 4 compounds. Flow cytometry analysis demonstrated that both compounds 3 and 6 caused cell cycle arrest at the G0/G1 phase and induced cellular Apoptosis in HCT116 cells. Compounds 3 and 6 modulated the expression levels of key signaling proteins closely related to cell proliferation and Apoptosis, i.e., increasing the levels of poly(ADP-ribose) polymerase, p53, and p27, and decreasing the levels of EGFR, cyclin D1, CDK2 and CDK4. Overall, our findings provided insight into the Anticancer components of Rubus corchorifolius L. f. leaves, which could facilitate their utilization as functional food ingredients.

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