2. Academic Validation
  3. Roburic Acid Suppresses NO and IL-6 Production via Targeting NF-κB and MAPK Pathway in RAW264.7 Cells

Roburic Acid Suppresses NO and IL-6 Production via Targeting NF-κB and MAPK Pathway in RAW264.7 Cells

  • Inflammation. 2017 Dec;40(6):1959-1966. doi: 10.1007/s10753-017-0636-z.
Yufen Chen 1 2,  Ning Ji 1,  Shunli Pan 1,  Zhe Zhang 1,  Ran Wang 1,  Yuling Qiu 1,  Meihua Jin 3,  Dexin Kong 4 5
Affiliations

Affiliations

  • 1 Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
  • 2 Pharmacy Department, Tanggu Hospital of Infectious Diseases of Tianjin Binhai New Area, Tianjin, 300454, China.
  • 3 Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China. [email protected].
  • 4 Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China. [email protected].
  • 5 Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. [email protected].
PMID: 28761990 DOI: 10.1007/s10753-017-0636-z
Abstract

In the present study, we investigated the anti-inflammatory effect of roburic acid on production of nitric oxide (NO) and interlukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. We found that roburic acid reduced production of NO and IL-6, and the expression of inducible nitric oxide synthases (iNOS). Meanwhile, phosphorylation of inhibitor of κBα (IκBα) and IκB kinase α/β (IKKα/β), as well as translocation of nuclear factor-κB (NF-κB) to the nucleus, was suppressed by roburic acid treatment. In addition, phosphorylation of mitogen-activated protein kinase (MAPKs) including p38 and c-Jun-NH2-terminal kinase (JNK) was inhibited. Roburic acid exhibited inhibitory activities on production of NO and IL-6 via blocking IKK/IκB/NF-κB and MAPKs pathway, suggesting the potential application as a drug candidate for therapy of inflammatory diseases.

Keywords

IL-6; MAPKs; NF-κB; NO; inflammation; roburic acid.

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