1. Academic Validation
  2. Jatrorrhizine reduces 5-HT and NE uptake via inhibition of uptake-2 transporters and produces antidepressant-like action in mice

Jatrorrhizine reduces 5-HT and NE uptake via inhibition of uptake-2 transporters and produces antidepressant-like action in mice

  • Xenobiotica. 2019 Oct;49(10):1237-1243. doi: 10.1080/00498254.2018.1542188.
Siyuan Sun 1 2 Sisi Zhou 1 Shaowei Lei 1 Shujie Zhu 1 Kai Wang 1 Huidi Jiang 1 Hui Zhou 1
Affiliations

Affiliations

  • 1 a Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences , Zhejiang University , Hangzhou , PR China.
  • 2 b Department of Dermatology and Venereology, School of Medicine , Sir Run Run Shaw Hospital, Zhejiang University , Hangzhou , PR China.
Abstract

1. Jatrorrhizine is an active ingredient found in various traditional Chinese medicinal Plants. Based on our previous finding that jatrorrhizine was a potent inhibitor of OCT2 and OCT3, the aim of the present study was to explore whether jatrorrhizine has an antidepressant-like action action via inhibition of uptake-2 transporters. 2. In vitro uptake tests showed that jatrorrhizine strongly inhibited PMAT-mediated MPP+ uptake with an IC50 value of 1.05 μM and reduced 5-HT and NE uptake mediated by hOCT2, hOCT3 and hPMAT with IC50 values of 0.1-1 μM (for OCT2 and OCT3) and 1-10 μM (for PMAT). 3. In mouse synaptosomes, jatrorrhizine suppressed 5-HT and NE uptake in a concentration dependently manner, where the role of uptake-2 inhibition is significant. 4. The antidepressant-like action of jatrorrhizine was evaluated by mouse tail suspension test (TST). The TST showed that one week of jatrorrhizine (5, 10 and 20 mg/kg, i.p.) or venlafaxine (20 mg/kg, i.g.) can significantly reduce the duration of immobility when compared with vehicle control group. 5. The concentration of jatrorrhizine shows a dose-dependent increase in brain tissues. 6. Our study suggested that jatrorrhizine might be used as an antidepressant agent via inhibition of uptake-2 transporters.

Keywords

Jatrorrhizine; antidepressant; organic cation transporter; plasma membrane monoamine transporter; uptake-2.

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