2. Academic Validation
  3. miR-34a alleviates spinal cord injury via TLR4 signaling by inhibiting HMGB-1

miR-34a alleviates spinal cord injury via TLR4 signaling by inhibiting HMGB-1

  • Exp Ther Med. 2019 Mar;17(3):1912-1918. doi: 10.3892/etm.2018.7102.
Jianmin Zhou 1,  Ou Shuang 1,  Jian Li 1,  Zijun Cai 1,  Chong Wu 1,  Wenyu Wang 1
Affiliations

Affiliation

  • 1 Department of Orthopedics, Shangrao People's Hospital, Shangrao, Jiangxi 334000, P.R. China.
PMID: 30783468 DOI: 10.3892/etm.2018.7102
Abstract

The aim of the present study was to investigate the effect of MicroRNA (miR)-34a on spinal cord injury (SCI)-induced inflammation and the possible underlying mechanisms. The results indicated that miR-34a expression was downregulated in a rat model of SCI compared with the control group. Furthermore, miR-34a knockdown was demonstrated to aggravate inflammation, inhibit cell proliferation and enhance Apoptosis in an in vitro model of SCI. MiR-34a inhibition was demonstrated to upregulate the expression of inducible nitric oxide synthase and nitric oxide, as well as inducing the expression of Toll-like Receptor 4 (TLR4) and high mobility group box-1 (HMGB-1) in an in vitro model of SCI. TLR4 Inhibitor reduced the effects of miR-34a downregulation on inflammation and cell growth in SCI. Together, these results suggest that miR-34a is able to alleviate SCI via inhibiting HMGB-1 expression in TLR4 signaling.

Keywords

high mobility group box 1; inflammation; microRNA-34a; spinal cord injury; toll-like receptor 4.

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