1. Academic Validation
  2. miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

  • J Endocrinol. 2020 Mar;244(3):459-471. doi: 10.1530/JOE-19-0331.
Xin Li 1 Hongjiao Li 1 2 Di Zhang 2 Guojin Xu 1 Jinglin Zhang 3 Sheng Cui 1 2 3 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
  • 2 College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
  • 3 Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
  • 4 Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, People's Republic of China.
Abstract

MicroRNA-7 (miR-7) is an important modulator of a plenty of gene expressions and the interrelated biological processes, highly expressed in porcine pituitary. Norepinephrine (NE), acting as an important neurotransmitter or/and a hormone secreted excessively under stress, affects the synthesis and secretion of various Hormones, including pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which are the key Hormones which regulate sexual maturation and reproductive functions. However, the relationship among NE, miR-7 and gonadotropin needs to be elucidated. The aim of this study was to identify whether miR-7 involved in the NE-adrenoceptor signaling pathway affects the synthesis and secretion of FSH and LH in porcine pituitary. Our results showed that the NE intracerebroventricular injection increased pituitary miR-7 level and the synthesis and secretion of FSH and LH in porcine, whereas the inhibition of either endogenous miR-7 or β-adrenergic receptors hindered the rise of FSH and LH synthesis induced by NE in cultured primary porcine anterior pituitary cells. Further, we identified the molecular type of β-adrenergic receptors and the signaling pathway in porcine pituitary, and we found that NE played its roles relying on adrenoceptor beta 2 (β2AR) and the Raf/MEK/ERK1/2 signaling pathway. The phosphorylation of ERK1/2 upregulated miR-7 level which subsequently enhanced FSH and LH synthesis by targeting to Golgi glycoprotein 1 (GLG1). These suggest that miR-7 mediates NE's effect on promoting FSH and LH synthesis in porcine pituitary.

Keywords

gonadotropin; miR-7; norepinephrine; pig; pituitary.

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