1. Academic Validation
  2. LRP4 LDLα repeats of astrocyte enhance dendrite arborization of the neuron

LRP4 LDLα repeats of astrocyte enhance dendrite arborization of the neuron

  • Mol Brain. 2020 Dec 10;13(1):166. doi: 10.1186/s13041-020-00708-z.
Min Yan 1 2 3 Amin Guo 1 3 Peng Chen 1 3 Hongyang Jing 1 3 Dongyan Ren 1 3 Yanzi Zhong 1 3 Yongqiang Wu 1 3 Erkang Fei 1 3 Xinsheng Lai 1 3 Suqi Zou 1 3 Shunqi Wang 4 5
Affiliations

Affiliations

  • 1 School of Life Sciences, Nanchang University, Nanchang, Jiangxi, China.
  • 2 School of Basic Medical Sciences, Nanchang University, Nanchang, China.
  • 3 Institute of Life Sciences, Nanchang University, Nanchang, Jiangxi, China.
  • 4 School of Life Sciences, Nanchang University, Nanchang, Jiangxi, China. [email protected].
  • 5 Institute of Life Sciences, Nanchang University, Nanchang, Jiangxi, China. [email protected].
Abstract

The low-density lipoprotein receptor-related protein 4 (LRP4) is essential for inducing the neuromuscular junction (NMJ) formation in muscle fibers, and LRP4 plays a critical role in dendritic development and synaptogenesis in the central nervous system (CNS). As a single transmembrane protein, LRP4 contains an enormously sizeable extracellular domain (ECD), containing multiple LDLα repeats in the N-terminal of ECD. LRP4 only with extracellular domain acts as a similar mechanism of full-length LRP4 in muscles to stimulate acetylcholine receptor clustering. In this study, we elucidated that LDLα repeats of LRP4 maintained the body weight and survival rate. Dendritic branches of the pyramidal neurons in Lrp4-null mice with LRP4 LDLα repeats residue were more than in Lrp4-null mice without residual LRP4 domain. Supplement with conditioned medium from LRP4 LDLα overexpression cells, the primary culture pyramidal neurons achieved strong dendritic arborization ability. Besides, astrocytes with LRP4 LDLα repeats residue could promote pyramidal neuronal dendrite arborization in the primary co-cultured system. These observations signify that LRP4 LDLα repeats play a prominent underlying role in dendrite arborization.

Keywords

Dendrite arborization; Golgi staining; LRP4; Ldlα repeats; Primary culture; Pyramidal neuron.

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