2. Academic Validation
  3. Small extracellular vesicle-mediated Hsp70 intercellular delivery enhances breast cancer adriamycin resistance

Small extracellular vesicle-mediated Hsp70 intercellular delivery enhances breast cancer adriamycin resistance

  • Free Radic Biol Med. 2021 Feb 20:164:85-95. doi: 10.1016/j.freeradbiomed.2020.12.436.
Weizi Hu 1,  Zhi Xu 2,  Shuyi Zhu 3,  Wenbo Sun 4,  Xiumei Wang 4,  Chunli Tan 2,  Yanyan Zhang 4,  Guangqin Zhang 5,  Yong Xu 6,  Jinhai Tang 7
Affiliations

Affiliations

  • 1 Laboratory of Cancer Biology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & the Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting, Nanjing, 210009, PR China; Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, PR China; Jingzhou Center Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 60 Jingzhon Middle Rd., Jingzhon, Hubei Province, PR China.
  • 2 Laboratory of Cancer Biology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & the Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting, Nanjing, 210009, PR China; Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, PR China.
  • 3 Laboratory of Cancer Biology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & the Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting, Nanjing, 210009, PR China; Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Nanjing Medical University, Nanjing, 211166, PR China.
  • 4 Laboratory of Cancer Biology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & the Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting, Nanjing, 210009, PR China.
  • 5 School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, 639 Longmian Road, Nanjing, 211198, PR China.
  • 6 Laboratory of Cancer Biology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & the Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting, Nanjing, 210009, PR China; Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Nanjing Medical University, Nanjing, 211166, PR China. Electronic address: [email protected].
  • 7 Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, PR China. Electronic address: [email protected].
PMID: 33418113 DOI: 10.1016/j.freeradbiomed.2020.12.436
Abstract

Adriamycin (ADR) resistance poses a significant challenge for successfully treating breast Cancer (BCa). The mechanism underlying intrinsically acquisition of the resistance remains to be fully elucidated. Here, we describe that small extracellular vesicles (sEVs) mediated HSP70 transfer is implicated in ADR resistance. The resistant cells derived sEVs were incubated with sensitive cells, thereby transmitting the resistant phenotype to the recipient cells. The internalization of the sEVs in the recipient cells and sEV-mediated HSP70 transfer into mitochondria were examined by confocal microscope and transmission electron microscopy (TEM). Oxygen consumption rate (OCR) incorporated with extracellular acidification rate (ECAR) was quantified by Seahorse XF Analyzer. Mechanistically, sEVs transported HSP70, leading to increased Reactive Oxygen Species (ROS) and impaired mitochondria in the recipient cells, thereby inhibiting respiration but promoting glycolysis. The sEVs effect on the metabolism of the recipient cells was alleviated by silencing HSP70 in sEVs donor cells. The aspect of sEV-Hsp70 on drug-resistant transmission was further validated by tumor zebrafish xenografts. The finding from this work suggests that sEV-mediated HSP70 intercellular delivery enhances ADR resistance mainly through reprogramming the recipient cell energy metabolism.

Keywords

ADR resistance and breast cancer; Hsp70; Reprogramming energy metabolism; Small extracellular vesicles.

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