1. Academic Validation
  2. MicroRNA profiling in Chinese children with Henoch-Schonlein purpura and association between selected microRNAs and inflammatory biomarkers

MicroRNA profiling in Chinese children with Henoch-Schonlein purpura and association between selected microRNAs and inflammatory biomarkers

  • Acta Paediatr. 2021 Jul;110(7):2221-2229. doi: 10.1111/apa.15789.
Jing Li 1 Meixue Chen 1 Jinfeng Wang 1 Lingling Lu 1 Xiang Li 1 Yuan Le 1
Affiliations

Affiliation

  • 1 Department of Pediatrics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Abstract

Aim: This study aimed to profile the MicroRNA levels in Chinese Henoch-Schonlein purpura (HSP) children and to explore their association with inflammatory factors and T helper 17 (Th17)/regulatory T (Treg).

Methods: Forty-five HSP children and 27 healthy controls were enrolled in this study, and MicroRNA levels were profiled with a MicroRNA microarray. The levels of selected MicroRNAs were determined by quantitative Real-Time PCR, and the levels of serum IgA, interleukin-6, interleukin-10 and interleukin-17A were detected by enzyme-linked immunosorbent assay. Additionally, Th17 and Treg cells were analysed by flow cytometry.

Results: There were 9 up-regulated and 27 down-regulated MicroRNAs in the PBMCs of Chinese HSP children. Among them, miR-1-3p, miR-19b-1-5p and miR-29b-1-5p were up-regulated, while miR-483-5p and miR-1246 were down-regulated. Additionally, these selected MicroRNAs could differentiate HSP patients from healthy controls. Interestingly, miR-29b-1-5p was correlated with IgA, miR-19b-1-5p, miR-483-5p and miR-1246 were correlated with interleukin-6, while miR-1-3p and miR-1246 were correlated with Th17/Treg.

Conclusion: This study reveals that the altered MicroRNAs could differentiate HSP from the healthy, and were associated with inflammatory factors or Th17/Treg. It is indicated that alteration in these MicroRNAs may contribute to the HSP pathogenesis and may become therapeutic targets or diagnostic biomarkers for HSP.

Keywords

Henoch-Schonlein purpura; IL-6; IgA; Th17/Treg; microRNA.

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