1. Academic Validation
  2. Long Non-coding RNA SENP3-EIF4A1 Functions as a Sponge of miR-195-5p to Drive Triple-Negative Breast Cancer Progress by Overexpressing CCNE1

Long Non-coding RNA SENP3-EIF4A1 Functions as a Sponge of miR-195-5p to Drive Triple-Negative Breast Cancer Progress by Overexpressing CCNE1

  • Front Cell Dev Biol. 2021 Mar 15;9:647527. doi: 10.3389/fcell.2021.647527.
Lie Chen 1 Xiaofei Miao 2 Chenchen Si 3 An Qin 1 Ye Zhang 2 Chunqiang Chu 1 Zengyao Li 2 Tong Wang 2 Xiao Liu 1
Affiliations

Affiliations

  • 1 Department of Thyroid and Breast Surgery, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, China.
  • 2 Department of General Surgery, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, China.
  • 3 Dermatological Department, Wuxi Children's Hospital Affiliated to Nanjing Medical University, Wuxi, China.
Abstract

Triple-negative breast Cancer (TNBC) has high malignancy and limited treatment, so novel molecular therapeutic targets are urgently needed. Cyclin E1 (CCNE1) promotes progression in breast Cancer, but its role and inherent mechanisms in TNBC are yet to be elucidated. Competing endogenous RNA (ceRNA) may be a potential mechanism. CCNE1 was selected though bioinformatics and clinical samples, and cell lines were utilized to verify CCNE1 expression by qRT-PCR and western blot. Predicting tools provided potential miR-195-5p and SENP3-EIF4A1 and tested from multilevel. Functional experiments were conducted in vitro and in vivo. Luciferase reporter assay and RNA immunoprecipitation experiments were implemented to ensure the interaction between miR-195-5p and SENP3-EIF4A1/CCNE1 in TNBC. Bioinformatics found DNA hypermethylation of miR-195-5p and preliminarily verified. Mechanistically, SENP3-EIF4A1-miR-195-5p-associated ceRNA could drive TNBC progress though regulating CCNE1. DNA hypermethylation of miR-195-5p might be another reason. In summary, SENP3-EIF4A1-miR-195-5p-CCNE1 axis promotes TNBC progress and may contribute to the novel diagnosis and treatment of TNBC.

Keywords

CCNE1; DNA methylation; LncRNA; microRNA; triple-negative breast cancer.

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