1. Epigenetics Apoptosis Cell Cycle/DNA Damage
  2. DNA Methyltransferase Apoptosis Nucleoside Antimetabolite/Analog
  3. Decitabine

Decitabine  (Synonyms: 5-Aza-2'-deoxycytidine; 5-AZA-CdR; NSC 127716)

Cat. No.: HY-A0004 Purity: 99.87%
COA Handling Instructions

Décitabine (NSC 127716) est un antimétabolite analogue de désoxycytidine et un ADN méthyltransférase. Décitabine incorporée dans l'ADN à la place de la cytosine peut piéger par covalence la ADN méthyltransférase dans l'ADN, provoquant une inhibition irréversible de l'enzyme. Décitabine induit un arrêt cellulaire G2/M et l'apoptose cellulaire. Décitabine a une activité puissante anticancéreuse.

Decitabin (5-Aza-2'-Desoxycytidin) ist ein deoxycytidine analogue antimetabolite und DNA methyltransferase-Inhibitor. Decitabin wird anstelle von Cytosin in die DNA eingebaut und kann die DNA methyltransferase kovalent an die DNA binden, was zu einer irreversiblen Hemmung des Enzyms führt. Decitabin induziert den G2/M-Anhalt der Zelle und die apoptosis. Decitabin hat eine starke krebsbekämpfende Wirkung.

Decitabine (NSC 127716) is an orally active deoxycytidine analogue antimetabolite and a DNA methyltransferase inhibitor. Decitabine incorporates into DNA in place of cytosine can covalently trap DNA methyltransferase to DNA causing irreversible inhibition of the enzyme. Decitabine induces cell G2/M arrest and cell apoptosis. Decitabine has potent anticancer activity.

For research use only. We do not sell to patients.

Decitabine Chemical Structure

Decitabine Chemical Structure

CAS No. : 2353-33-5

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
100 mg USD 50 In-stock
500 mg USD 150 In-stock
1 g USD 250 In-stock
2 g USD 450 In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 91 publication(s) in Google Scholar

Top Publications Citing Use of Products

77 Publications Citing Use of MCE Decitabine

RT-PCR
WB
Proliferation Assay
IF

    Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The mRNA expression of GSDME is detected using RT-PCR after treatment with different concentrations of Decitabine for 3 days (the cells were centrifuged, and decitabine is replaced every day).

    Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The protein level of GSDME in cells is quantitatively analysed by western blot in Y79 and WERI-RB-1 cells after Decitabine (2 nM; 3 days) treatment.

    Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The protein level of GSDME in cells is detected using immunofluorescence in Y79 and WERI-RB-1 cells after Decitabine (2 nM; 3 days) treatment.

    Decitabine purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The toxicity of decitabine to Y79 and WERI-RB-1 cells is detected by CCK-8 assay at different concentrations of Decitabine.

    Decitabine purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 20;11(11):5605-5619.  [Abstract]

    The mRNA levels of PCK2 in RCC cell lines after 5-AZA treatment. PCK2 protein expression in RCC cell lines after 5-AZA treatment.

    Decitabine purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 May 24;e13072.  [Abstract]

    The indicated HSCs are subjected to DNMTs inhibitor (5‐azadC, 2 μM), G9a inhibitor (UNC0642, 2 μM) and pan‐HDAC inhibitor (ITF‐2357, 100 nM) for 48 h, or EZH2 inhibitor (EPZ‐6438, 10 μM) for 72h. KLF14 expression is measured by Western blotting analyses.

    Decitabine purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 May 24;e13072.  [Abstract]

    The indicated HSCs are subjected to DNMTs inhibitor (5‐azadC, 2 μM), G9a inhibitor (UNC0642, 2 μM) and pan‐HDAC inhibitor (ITF‐2357, 100 nM) for 48 h, or EZH2 inhibitor (EPZ‐6438, 10 μM) for 72h. KLF14 expression is measured by RT‐qPCR.

    Decitabine purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2021 Feb 23;102028.  [Abstract]

    The TF changes induced by DNMT3L in these cells are not affected with Decitabine (10 µM; for 24 hours) treatment in most genes tested, except NEUROD2 and NEUROG2 as shown by qRTPCR.

    Decitabine purchased from MedChemExpress. Usage Cited in: Oncogene. 2020 Aug;39(33):5507-5519.  [Abstract]

    Quantitative real-time PCR analysis of CDH13 expression after 5 μM Decitabine treatment.

    Decitabine purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2020 Dec;140(12):2442-2454.e5.  [Abstract]

    In EZH2 mutant cells, after six days of drug treatment with both GSK126 and 0.5 μM Decitabine, Significant upregulation of RASSF5, EPAS1, ITGB2, and NDRG1 target gene expression is seen in EZH2 mutant cell lines.

    Decitabine purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2020 Dec;140(12):2442-2454.e5.  [Abstract]

    In EZH2 mutant cells, after six days of drug treatment with both GSK126 and 0.5 μM Decitabine, Western blots shows complete elimination of H3K37me3 with reductions in DNMT1A, DNMT3A, and DNMT3B.

    Decitabine purchased from MedChemExpress. Usage Cited in: Ann Rheum Dis. 2019 Oct;78(10):1420-1429.  [Abstract]

    Western blot of PPARγ, MMP13, Aamts5, Aggrecan and Collagen2 from Sham, DMM and 5Aza-treated mouse joint tissues ( (0.35 mg/kg; intraperitoneal injection daily; 10 weeks).

    Decitabine purchased from MedChemExpress. Usage Cited in: Ann Rheum Dis. 2019 Oct;78(10):1420-1429.  [Abstract]

    Western blot of PPARγ from mouse chondrocytes treated with IL-1β (10 ng/mL) for 24 hours in absence or presence 5Aza of various doses (0, 1, 5 and 10 µM).

    Decitabine purchased from MedChemExpress. Usage Cited in: J Allergy Clin Immunol. 2019 Jun;143(6):2038-2051.e12.  [Abstract]

    Naive CD41 T cells are stimulated with anti-CD3/CD28 in the presence of mock stimulant or Decitabine (5-aza-29-deoxycytidine; 1 µM). Real-time PCR analysis of Il4 and Il13 mRNA after 24 hours of stimulation and IL-4 and IL-13 concentrations in culture supernatants after 72 hours of stimulation are measured.

    View All DNA Methyltransferase Isoform Specific Products:

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Decitabine (NSC 127716) is an orally active deoxycytidine analogue antimetabolite and a DNA methyltransferase inhibitor. Decitabine incorporates into DNA in place of cytosine can covalently trap DNA methyltransferase to DNA causing irreversible inhibition of the enzyme. Decitabine induces cell G2/M arrest and cell apoptosis. Decitabine has potent anticancer activity[1][2].

    IC50 & Target[5]

    DNMT1

     

    DNMT3A

     

    DNMT3B

     

    In Vitro

    Decitabine treatment significantly inhibits cell growth of SNU719, NCC24 and KATOIII 96 hours after exposure to decitabine. Decitabine induces G2/M arrest and apoptosis in EBVaGC, inhibits invasion ability, and up-regulates E-cadherin expression for EBVaGC[1].
    ? Only high concentrations (10 μM) Decitabine (0.1-1 μM; 24-72 hours) results in a G2 phase arrest, which is accompanied by a reduction of cells in G1 phase[3].
    ? Decitabine up-regulates DCTPP1 and dUTPase expression in HeLa cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Cycle Analysis[1]

    Cell Line: HCT116 cells
    Concentration: 0.1, 1, 10 µM
    Incubation Time: 24, 48, 72 hours
    Result: Only high drug concentrations (10 µM) resulted in a G2 phase arrest, which was accompanied by a reduction of cells in G1 phase.
    In Vivo

    Decitabine (1.0 mg/kg, p.o.) in combination with tetrahydrouridine (THU) causes severe toxicity occurs in female CD-1 mice, and results in an increased sensitivity to decitabine toxicity correlating with decitabine plasma levels[5].
    ? Decitabine (1.0 mg/kg; i.p.; once daily for 5 consecutive days) leads to regression of EL4 tumors established in C57BL/6 Mice[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6 mice (bearing EL4 cells)[6]
    Dosage: 1.0 mg/kg
    Administration: Intraperitoneal injection; once daily for 5 consecutive days
    Result: Caused continuous tumor regression even after Decitabine treatment was stopped.
    Clinical Trial
    Molecular Weight

    228.21

    Formula

    C8H12N4O4

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1C[C@H](N2C(N=C(N)N=C2)=O)O[C@@H]1CO

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 1 years; -20°C, 6 months (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (219.10 mM)

    H2O : 20 mg/mL (87.64 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3819 mL 21.9096 mL 43.8193 mL
    5 mM 0.8764 mL 4.3819 mL 8.7639 mL
    10 mM 0.4382 mL 2.1910 mL 4.3819 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 10 mg/mL (43.82 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.97%

    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email Address *

    Phone Number *

     

    Organization Name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Decitabine
    Cat. No.:
    HY-A0004
    Quantity:
    MCE Japan Authorized Agent: