2. Academic Validation
  3. Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

  • Foods. 2021 Dec 20;10(12):3155. doi: 10.3390/foods10123155.
Lijia Zhang 1,  Mengzhou Yin 1,  Xi Feng 2,  Salam A Ibrahim 3,  Ying Liu 1,  Wen Huang 1
Affiliations

Affiliations

  • 1 College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
  • 2 Department of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USA.
  • 3 Department of Family and Consumer Sciences, North Carolina Agricultural and Technical State University, 171 Carver Hall, Greensboro, NC 27411, USA.
PMID: 34945705 DOI: 10.3390/foods10123155
Abstract

In this study, triterpenoid compounds from Poriae Cutis were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-α, IL-1β, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 μg/mL. The results provide evidence for the use of Poriae Cutis as a natural anti-inflammatory agent in medicines and functional foods.

Keywords

Poriae Cutis; anti-inflammatory; identification; purification; triterpenoid.

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