2. Academic Validation
  3. Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway

Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway

  • Pharmaceuticals (Basel). 2022 Jan 6;15(1):73. doi: 10.3390/ph15010073.
Sang-Hyun Ahn 1,  Jung-Soo Suh 1,  Yoon-Kwan Jang 1,  Heon-Su Kim 1,  Gyu-Ho Choi 1,  Eunhye Kim 1,  Tae-Jin Kim 1 2 3
Affiliations

Affiliations

  • 1 Department of Integrated Biological Science, Pusan National University, Pusan 46241, Korea.
  • 2 Department of Biological Sciences, Pusan National University, Pusan 46241, Korea.
  • 3 Institute of Systems Biology, Pusan National University, Pusan 46241, Korea.
PMID: 35056130 DOI: 10.3390/ph15010073
Abstract

Rhynchosia volubilis, a small black bean, has been used as a traditional remedy to treat diseases and maintain health in East Asia, but its cellular effects and molecular mechanisms are not fully understood. The purpose of this study was to investigate the effect of ethanol extract from Rhynchosia volubilis (EERV) on cell survival and to elucidate the biochemical signaling pathways. Our results showed that EERV stimulated the cyclic AMP (cAMP) signal revealed by a fluorescent protein (FP)-based intensiometric sensor. Using a Förster resonance energy transfer (FRET)-based sensor, we further revealed that EERV could activate PKA and ERK signals, which are downstream effectors of cAMP. In addition, we reported that EERV could induce the phosphorylation of CREB, a key signal for cell survival. Thus, our results suggested that EERV protects against Apoptosis by activating the cell survival pathway through the cAMP-PKA/ERK-CREB pathway.

Keywords

CREB; FRET; PKA; Rhynchosia volubilis; cAMP.

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