1. GPCR/G Protein
  2. Adrenergic Receptor
  3. Isoprenaline hydrochloride

Isoprenaline hydrochloride (Synonyms: Isoproterenol hydrochloride)

Cat. No.: HY-B0468 Purity: >99.0%
Handling Instructions

Isoprenaline hydrochloride is a non-selective beta-adrenergic receptor agonist with potent peripheral vasodilator, bronchodilator, and cardiac stimulating activities.

For research use only. We do not sell to patients.

Isoprenaline hydrochloride Chemical Structure

Isoprenaline hydrochloride Chemical Structure

CAS No. : 51-30-9

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10 mM * 1 mL in DMSO USD 53 In-stock
Estimated Time of Arrival: December 31
200 mg USD 48 In-stock
Estimated Time of Arrival: December 31
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Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Isoprenaline hydrochloride purchased from MCE. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2018 Dec;391(12):1373-1385.

    NRCMs are pre-incubated with PCA (50, 100, and 200 μM) for 1 h followed by 10 μM Isoproterenol (ISO) treatment. Cell surface area is measured by rhodamine-phalloidin staining. The expression of β-MHC is detected by Western blot.

    Isoprenaline hydrochloride purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2019 Apr 25;38(1):174. 

    EHD1 stabilizes β2AR. A549 cells are incubated in serum-free 1640 medium for 16 h and then treated with ISO (10 μM) for the indicated times in the presence of cycloheximide (CHX, 20 μg/mL). The cell lysates are analyzed by Western blot.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Isoprenaline hydrochloride is a non-selective beta-adrenergic receptor agonist with potent peripheral vasodilator, bronchodilator, and cardiac stimulating activities.

    IC50 & Target

    Beta-adrenergic receptor[1]

    In Vitro

    Isoprenaline (300 nM, 3 min) increases particulate cGMP- and cilostamide-inhibited, low-Km cAMP phosphodiesterase (cAMP-PDE) activity by about 100% in intact rat fat cells[1]. Isoprenaline inhibits insulin-stimulated glucose transport activity in rat adipocytes. Isoprenaline, in the absence of adenosine, promotes a time-dependent (t1/2 approximately 2 min) decrease in the accessibility of insulin-stimulated cell surface GLUT4 of > 50%, which directly correlated with the observed inhibition of transport activity[2]. Isoprenaline (5 nM and 10 mM) increases cyclic AMP levels and this effect is potentiated by cilostamide (10 mM), by rolipram, a cyclic AMP-specific PDE (PDE 4) inhibitor (10 mM) and by cyclic GMP-elevating agents (50 nM ANF or 30 nM SNP plus 100 nM DMPPO)[3]. Isoprenaline increases the transcriptional activity of Gi alpha-2 gene to 140% of the control value, whereas gene specific hybridization for Gs alpha remains unchanged[4]. Isoprenaline (20 nM) increases the amplitude of total iK and causes a negative shift of approximately 10 mV in the activation curve for iK, both in the absence and in the presence of 300 nM nisoldipine to block the L-type Ca2+ current. Isoprenaline (20 nM) increases the spontaneous pacemaker rate of sino-atrial node pacemaker cells by 16% in rabbit isolated pacemaker cells[5].

    Storage

    4°C, protect from light

    Solvent & Solubility
    In Vitro: 

    DMSO : 80 mg/mL (322.95 mM; Need ultrasonic)

    H2O : ≥ 50 mg/mL (201.84 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.0368 mL 20.1841 mL 40.3682 mL
    5 mM 0.8074 mL 4.0368 mL 8.0736 mL
    10 mM 0.4037 mL 2.0184 mL 4.0368 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [3]

    Cells are seeded in 24-well culture dishes at a density of 2 to 5×104 cells per well. Experiments are performed after 3 to 5 days in culture when cells has reached confluence. Culture medium is aspirated and replaced by 0.5 mL of PBS containing the pharmacological agents. Treatments are performed in quadruplicate at 37°C. The type 3, 4 and 5 PDE inhibitors cilostamide (10 gM), rolipram (10 pM) and DMPPO (10 gM), respectively, are incubated with cells for 30 min before addition of adenylate or guanylate cyclase activators. Cyclic GMP and cyclic AMP are respectively increased in RASMC by stimulation of particulate guanylate cyclase with ANF (50 nM for 10 min) or fl-adrenoceptors with isoprenaline (5 nm for 5 min). At the end of the incubation period, the medium is removed and intracellular cyclic nucleotides are extracted by two ethanolic (65%) ishes at 4°C for 5 min. Ethanolic extracts are pooled, evaporated to dryness by a Speed-Vac system. The dried extract is dissolved in a suitable amount of assay buffer and cyclic nucleotide levels are measured by scintillation proximity assay.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    247.72

    Formula

    C₁₁H₁₈ClNO₃

    CAS No.

    51-30-9

    SMILES

    OC1=CC=C(C(O)CNC(C)C)C=C1O.Cl

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: >99.0%

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    Product Name:
    Isoprenaline hydrochloride
    Cat. No.:
    HY-B0468
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    Isoprenaline hydrochloride

    Cat. No.: HY-B0468