Isoprenaline hemisulfate

Name: Isoprenaline hemisulfate
Brand: MedChemExpress
SKU: HY-108353A
Rating: 5.0 (87 reviews)

Cat. No.: HY-108353A Purity: 98.10%: Data Sheet
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Isoprenaline hemisulfate is a non-selective, orally active β-adrenergic receptor agonist. Isoprenaline has potent peripheral vasodilator, bronchodilator, and cardiac stimulating activities. Isoprenaline can be used for the research of bradycardia and bronchial asthma.

For research use only. We do not sell to patients.

CAS No. : 299-95-6

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 87 publication(s) in Google Scholar

Other Forms of Isoprenaline hemisulfate:

Isoprenaline In-stock
Isoprenaline hydrochloride In-stock

87 Publications Citing Use of MCE Isoprenaline hemisulfate

RT-PCR

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Sep 1.; Isoprenaline administration significantly reduces [email protected] anti-inflammatory and antioxidant activities.

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2024 Sep 1.; Isoprenaline significantly activates ERK1/2-induced DRP1 phosphorylation on serine 616 (pDRP1).

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]; Isoprenaline hydrochloride (ISO; 10 μM; 6 h), markedly inhibits SeV- or HSV-1-induced transcription of the IFNB1 and CXCL10 genes in THP1 cells.

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]; Isoprenaline hydrochloride (ISO; 10 μM; 0, 3, 6, 9 h) inhibits the phosphorylation of MITA^S366, IRF3^S386 and STAT1^Y701 induced by HSV-1, HSV-2 and HCMV, as well as the phosphorylation of IRF3^S386 and STAT1^Y701 induced by SeV, EMCV and VSV, in THP1 cells.

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2023 Apr:174:113670. [Abstract]; Isoprenaline hydrochloride (ISO; 10 μM; 6 h) pretreatment markedly restores the ATGL and HSL expressions decreased by 1,3-dichloro-2-propanol (1,3-DCP; 100 μM; 6 h) in HepG2 cells.

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Mar;30(3):766-778. [Abstract]; SVF differentiated adipocytes were treated with 2 μM isoprenaline (ISO) for 12h followed by Q-PCR analysis of browning genes (n=3). Gene expression is normalized to the 36B4 endogenous control.

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Apr 25;38(1):174. [Abstract]; EHD1 stabilizes β2AR. A549 cells are incubated in serum-free 1640 medium for 16 h and then treated with ISO (10 μM) for the indicated times in the presence of cycloheximide (CHX, 20 μg/mL). The cell lysates are analyzed by Western blot.

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2019 Apr 25;38(1):174. [Abstract]; A549 cells were transfected with vector or ShEHD1 and then treated with DMSO or ISO (Isoprenaline) (10 μM; 16 h), and the EHD1 and VEGFA protein levels were assessed by immunoblotting.

: Isoprenaline hemisulfate purchased from MedChemExpress. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2018 Dec;391(12):1373-1385. [Abstract]; NRCMs are pre-incubated with PCA (50, 100, and 200 μM) for 1 h followed by 10 μM Isoproterenol (ISO) treatment. Cell surface area is measured by rhodamine-phalloidin staining. The expression of β-MHC is detected by Western blot.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

β adrenergic receptor

In Vitro

Isoprenaline hemisulfate (300 nM, 3 min) increases particulate cGMP- and cilostamide-inhibited, low-K_m cAMP phosphodiesterase (cAMP-PDE) activity by about 100% in intact rat fat cells^[1].
Isoprenaline inhibits insulin-stimulated glucose transport activity in rat adipocytes. Isoprenaline, in the absence of adenosine, promotes a time-dependent (t1/2 approximately 2 min) decrease in the accessibility of insulin-stimulated cell surface GLUT4 of > 50%, which directly correlated with the observed inhibition of transport activity^[2].
Isoprenaline (5 nM and 10 μM) increases cyclic AMP levels and this effect is potentiated by cilostamide (10 mM), by rolipram, a cyclic AMP-specific PDE (PDE 4) inhibitor (10 mM) and by cyclic GMP-elevating agents (50 nM ANF or 30 nM SNP plus 100 nM DMPPO)^[3].
Isoprenaline increases the transcriptional activity of Gi alpha-2 gene to 140% of the control value, whereas gene specific hybridization for Gs alpha remains unchanged^[4].
Isoprenaline (20 nM) increases the amplitude of total iK and causes a negative shift of approximately 10 mV in the activation curve for iK, both in the absence and in the presence of 300 nM nisoldipine to block the L-type Ca²⁺ current^[5].
Isoprenaline (20 nM) increases the spontaneous pacemaker rate of sino-atrial node pacemaker cells by 16% in rabbit isolated pacemaker cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Isoprenaline hemisulfate (oral, 0.27-0. 64 μg/kg) is extensively metabolizes by a relatively small number of reactions in dogs^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Dogs^[1]
Dosage:	0.27-0. 64 μg/kg
Administration:	oral
Result:	Excreted largely unchanged in urine, only one-third of the radioactivity in urine was in the form of the O-methyl metabolite. Showed plasma radioactivity was almost entirely as conjugated isoprenaline and this metabolite accounted for more than 80% of radioactivity in urine. . Showed heart rate returned to base-line values when high plasma concentrations.

Molecular Weight

260.30

Formula

C₁₁H₁₇NO₃.1/2H₂O₄S

CAS No.

299-95-6

Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(C(O)CNC(C)C)C=C1O.O=S(O)(O)=O.[0.5]

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (384.17 mM; Need ultrasonic)

DMSO : 100 mg/mL (384.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8417 mL	19.2086 mL	38.4172 mL
5 mM	0.7683 mL	3.8417 mL	7.6834 mL
10 mM	0.3842 mL	1.9209 mL	3.8417 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.10%

Select Batch:

Data Sheet (282 KB) SDS (254 KB)

COA (253 KB) HNMR (184 KB) LCMS (333 KB)

Handling Instructions (2659 KB)

References

[1]. Degerman E, et al. Evidence that insulin and isoprenaline activate the cGMP-inhibited low-K_m cAMP phosphodiesterase in rat fat cells by phosphorylation. Proc Natl Acad Sci U S A. 1990 Jan;87(2):533-7. [Content Brief]

[2]. Vannucci SJ, et al. Cell surface accessibility of GLUT4 glucose transporters in insulin-stimulated rat adipose cells. Modulation by isoprenaline and adenosine. Biochem J. 1992 Nov 15;288 (Pt 1):325-30. [Content Brief]

[3]. Delpy E, et al. Effects of cyclic GMP elevation on isoprenaline-induced increase in cyclic AMP and relaxation in rat aortic smooth muscle: role of phosphodiesterase 3. Br J Pharmacol. 1996 Oct;119(3):471-8. [Content Brief]

[4]. Muller FU, et al. Isoprenaline stimulates gene transcription of the inhibitory G protein alpha-subunit Gi alpha-2 in rat heart. Circ Res. 1993 Mar;72(3):696-700. [Content Brief]

[5]. Lei M, et al. Modulation of delayed rectifier potassium current, iK, by isoprenaline in rabbit isolated pacemaker cells. Exp Physiol. 2000 Jan;85(1):27-35. [Content Brief]

[6]. M E Conolly, et al. Metabolism of isoprenaline in dog and man. Br J Pharmacol [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.8417 mL	19.2086 mL	38.4172 mL	96.0430 mL
	5 mM	0.7683 mL	3.8417 mL	7.6834 mL	19.2086 mL
	10 mM	0.3842 mL	1.9209 mL	3.8417 mL	9.6043 mL
	15 mM	0.2561 mL	1.2806 mL	2.5611 mL	6.4029 mL
	20 mM	0.1921 mL	0.9604 mL	1.9209 mL	4.8022 mL
	25 mM	0.1537 mL	0.7683 mL	1.5367 mL	3.8417 mL
	30 mM	0.1281 mL	0.6403 mL	1.2806 mL	3.2014 mL
	40 mM	0.0960 mL	0.4802 mL	0.9604 mL	2.4011 mL
	50 mM	0.0768 mL	0.3842 mL	0.7683 mL	1.9209 mL
	60 mM	0.0640 mL	0.3201 mL	0.6403 mL	1.6007 mL
	80 mM	0.0480 mL	0.2401 mL	0.4802 mL	1.2005 mL
	100 mM	0.0384 mL	0.1921 mL	0.3842 mL	0.9604 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.