1. Academic Validation
  2. Combined Effects of 2-Methoxyestradiol (Hypoxia-Inducible Factor 1 α Inhibitor) and Dasatinib (A Second-Generation Tyrosine Kinase Inhibitor) on Chronic Myelocytic Leukemia Cells

Combined Effects of 2-Methoxyestradiol (Hypoxia-Inducible Factor 1 α Inhibitor) and Dasatinib (A Second-Generation Tyrosine Kinase Inhibitor) on Chronic Myelocytic Leukemia Cells

  • J Immunol Res. 2022 Apr 28;2022:6324326. doi: 10.1155/2022/6324326.
Yuexian Zhang 1 Heng Chen 2 Yunfeng Shen 2 Xin Zhou 2
Affiliations

Affiliations

  • 1 Department of Hematology, Wuxi Branch of Ruijin Hospital, Wuxi, China.
  • 2 Department of Hematology, Wuxi People's Hospital, Wuxi, China.
Abstract

Chronic myelocytic leukemia (CML) is a frequently encountered type of leukemia in China. Hypoxia-inducible factor 1 (HIF-1) serves as one of the most important factors of oxygen balance transcription. The activation of this gene mostly marks a poor outlook for Cancer patients. To clarify the therapeutic effect of inhibiting this gene on CML, the present study is aimed at exploring the treatment effects of 2-methoxyestradiol (2-ME2), dasatinib alone, and combined both on K-562 cells and the possible mechanism of 2-ME2 in treating the disorder. The levels of HIF-1α, vascular endothelial growth factor (VEGF), and glutamate synthase 1 (GLU1) genes in K-562 cells were affected dose-dependently after 2-ME2 administration. 2-ME2 induced cell Apoptosis by downregulating antiapoptotic protein expressions of Bcl-xL and Bcl-2. The therapeutic effect of single 2-ME2 was superior to single dasatinib, and the effect of combined therapy of both drugs produced better effectiveness than either of the single drug. Once the concentration of 2-ME2 exceeded 0.5 μM, downregulated c-Myc gene expression could exert roles in anti-CML cell proliferation and inducing Apoptosis. Dasatinib might participate in the inhibition of the c-Myc pathway during this process whereas its effect remained not clear. Taken together, abnormal high expression of HIF-1α exerted an essential role in CML occurrence and development. Inhibition of this gene could markedly increase cell Apoptosis in a dose-dependent fashion. Moreover, 2-ME2 could induce cell Apoptosis by downregulating the c-Myc gene and exert an apoptotic effect by downregulating Bcl-xL and Bcl-2 which act as antiapoptotic proteins.

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