2. Academic Validation
  3. TGF-β1-induced bone marrow mesenchymal stem cells (BMSCs) migration via histone demethylase KDM6B mediated inhibition of methylation marker H3K27me3

TGF-β1-induced bone marrow mesenchymal stem cells (BMSCs) migration via histone demethylase KDM6B mediated inhibition of methylation marker H3K27me3

  • Cell Death Discov. 2022 Jul 28;8(1):339. doi: 10.1038/s41420-022-01132-z.
Qiting He 1 2,  Jie Shi 1,  Wei Liu 1,  Wei Zhao 1,  Zihao Wang 1,  Kaiwen Liu 1,  Dawang Zhao 1,  Shaoyi Wang 1,  Yongyuan Guo 1,  Lei Cheng 3,  Yuan Gao 4
Affiliations

Affiliations

  • 1 Department of Orthopedic Surgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong, China.
  • 2 NHC Key Laboratory of Otorhinolaryngology, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong, China.
  • 3 Department of Orthopedic Surgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong, China. [email protected].
  • 4 Department of Orthopedic Surgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong, China. [email protected].
PMID: 35902563 DOI: 10.1038/s41420-022-01132-z
Abstract

Mesenchymal stem cells (MSCs) are widely used in clinical research and therapy. Since the number of MSCs migration is extremely crucial at the lesion site, exploring the mechanisms to enhance the migration of MSCs is necessary. Therefore, this study focused on the epigenetic mechanisms in MSCs migration. TGF-β1 stimulated bone marrow mesenchymal stem cells (BMSCs) to promote cell migration at lesion sites in vitro and in vivo. The mRNA and protein levels of several migration-related genes (N cadherin, CXCR4, FN1) were enhanced. The trimethylation marker H3K27me3 recruitment on the promoter of these genes were studied to dissect the epigenetic mechanisms. TGF-β1 elevated the levels of KDM6B leading to removal of repression marker H3K27me3 in the promoter region of N Cadherins and FN1. Congruently, knockdown of demethylase KDM6B substantially affected the TGF-β1 induced BMSCs migration. This promoted the down-regulation of various migration-related genes. Collectively, epigenetic regulation played an important role in BMSCs migration, and H3K27me3 was at least partially involved in the migration of BMSCs induced by TGF-β1.

Figures
Products