2. Academic Validation
  3. 6'-O-Galloylpaeoniflorin attenuates Helicobacter pylori-associated gastritis via modulating Nrf2 pathway

6'-O-Galloylpaeoniflorin attenuates Helicobacter pylori-associated gastritis via modulating Nrf2 pathway

  • Int Immunopharmacol. 2022 Oct:111:109122. doi: 10.1016/j.intimp.2022.109122.
Yun Zhang 1,  Maihuan Wang 2,  Kebin Zhang 3,  Junze Zhang 4,  Xinpu Yuan 2,  Guijun Zou 2,  Zhen Cao 5,  Chaojun Zhang 6
Affiliations

Affiliations

  • 1 Department of General Surgery, School of Medicine, South China University of Technology, Guangzhou 510006, China; Department of General Surgery, The Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048, China.
  • 2 Department of General Surgery, The First Medical Center of Chinese, PLA General Hospital, Beijing 100853, China.
  • 3 Clinical Medical Research Center, Xinqiao Hospital, Army Medical University, Chongqing 400037, China.
  • 4 The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China; Department of General Surgery, The Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048, China.
  • 5 Department of General Surgery, The First Medical Center of Chinese, PLA General Hospital, Beijing 100853, China. Electronic address: [email protected].
  • 6 Department of General Surgery, School of Medicine, South China University of Technology, Guangzhou 510006, China; Department of General Surgery, The First Medical Center of Chinese, PLA General Hospital, Beijing 100853, China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China; Department of General Surgery, The Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048, China. Electronic address: [email protected].
PMID: 35964411 DOI: 10.1016/j.intimp.2022.109122
Abstract

As a common disease of the digestive system, chronic gastritis is inflammation of the gastric mucosa caused by various factors. Helicobacter pylori (H. pylori) is one of the main causes of chronic gastritis, which can lead to gastric mucosal damage and gland atrophy, thereby promoting gastrocarcinogenesis. Oxidative stress and the inflammatory response are important mechanisms of H. pylori-induced gastritis. 6'-O-Galloylpaeoniflorin (GPF) is a substance isolated from peony root with antioxidant and anti-inflammatory activities. However, its role and mechanism in the pathogenesis of H. pylori-induced chronic gastritis remain unclear. This study explored the effects of GPF on H. pylori-induced gastric mucosal oxidative stress and inflammation using flow cytometry, western blotting, real-time quantitative PCR, and immunohistochemistry. We found that H. pylori Infection increased oxidative stress and expression of inflammatory cytokines in vitro and in vivo and that these outcomes were inhibited by GPF. Furthermore, GPF activated nuclear factor erythroid-related factor-2 (Nrf2) and its downstream target genes in H. pylori-infected GES-1 cells and mice. The anti-inflammatory and antioxidant effects of GPF on H. pylori-infected cells were attenuated by an Nrf2 inhibitor. Taken together, these data suggest that GPF reduces H. pylori-induced gastric mucosa injury by activating Nrf2 signaling and that GPF is a potential candidate for the treatment of H. pylori-associated gastritis.

Keywords

6′-O-Galloylpaeoniflorin; Chronic gastritis; Helicobacter pylori; Inflammation; Nrf2; Oxidative stress.

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