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  2. Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis

Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis

  • Nat Commun. 2022 Sep 10;13(1):5324. doi: 10.1038/s41467-022-33063-9.
Marie Bobowski-Gerard 1 Clémence Boulet 1 Francesco P Zummo 1 Julie Dubois-Chevalier 1 Céline Gheeraert 1 Mohamed Bou Saleh 2 Jean-Marc Strub 3 Amaury Farce 2 Maheul Ploton 1 Loïc Guille 1 Jimmy Vandel 1 Antonino Bongiovanni 4 Ninon Very 1 Eloïse Woitrain 1 Audrey Deprince 1 Fanny Lalloyer 1 Eric Bauge 1 Lise Ferri 1 Line-Carolle Ntandja-Wandji 2 Alexia K Cotte 1 Corinne Grangette 5 Emmanuelle Vallez 1 Sarah Cianférani 3 Violeta Raverdy 6 Robert Caiazzo 6 Viviane Gnemmi 7 Emmanuelle Leteurtre 7 Benoit Pourcet 1 Réjane Paumelle 1 Kim Ravnskjaer 8 9 Guillaume Lassailly 2 Joel T Haas 1 Philippe Mathurin 2 François Pattou 6 Laurent Dubuquoy 2 Bart Staels 1 Philippe Lefebvre 1 Jérôme Eeckhoute 10
Affiliations

Affiliations

  • 1 Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011-EGID, F-59000, Lille, France.
  • 2 Univ. Lille, Inserm, CHU Lille, U1286 - INFINITE - Institute for Translational Research in Inflammation, F-59000, Lille, France.
  • 3 Laboratoire de Spectrométrie de Masse BioOrganique, CNRS UMR7178, Univ Strasbourg, IPHC, Infrastructure Nationale de Protéomique ProFI - FR2048, 67087, Strasbourg, France.
  • 4 Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41 - UAR 2014 - PLBS, F-59000, Lille, France.
  • 5 U1019-UMR 9017-CIIL-Centre d'Infection et d'Immunité de Lille, Institut Pasteur de Lille, Université de Lille, CNRS, Inserm, CHU Lille, F-59000, Lille, France.
  • 6 Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1190-EGID, Translational Research in Diabetes, Lille, France.
  • 7 Service d'anatomopathologie, Centre Hospitalier Universitaire de Lille, Université de Lille, INSERM UMR-S 1172, Lille, France.
  • 8 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, 5230, Denmark.
  • 9 Center for Functional Genomics and Tissue Plasticity (ATLAS), University of Southern Denmark, Odense, 5230, Denmark.
  • 10 Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011-EGID, F-59000, Lille, France. [email protected].
Abstract

Tissue injury triggers activation of mesenchymal lineage cells into wound-repairing myofibroblasts, whose unrestrained activity leads to fibrosis. Although this process is largely controlled at the transcriptional level, whether the main transcription factors involved have all been identified has remained elusive. Here, we report multi-omics analyses unraveling Basonuclin 2 (BNC2) as a myofibroblast identity transcription factor. Using liver fibrosis as a model for in-depth investigations, we first show that BNC2 expression is induced in both mouse and human fibrotic livers from different etiologies and decreases upon human liver fibrosis regression. Importantly, we found that BNC2 transcriptional induction is a specific feature of myofibroblastic activation in fibrotic tissues. Mechanistically, BNC2 expression and activities allow to integrate pro-fibrotic stimuli, including TGFβ and Hippo/YAP1 signaling, towards induction of matrisome genes such as those encoding type I collagen. As a consequence, Bnc2 deficiency blunts collagen deposition in livers of mice fed a fibrogenic diet. Additionally, our work establishes BNC2 as potentially druggable since we identified the thalidomide derivative CC-885 as a BNC2 inhibitor. Altogether, we propose that BNC2 is a transcription factor involved in canonical pathways driving myofibroblastic activation in fibrosis.

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