2. Academic Validation
  3. Syringin exerts anti-inflammatory and antioxidant effects by regulating SIRT1 signaling in rat and cell models of acute myocardial infarction

Syringin exerts anti-inflammatory and antioxidant effects by regulating SIRT1 signaling in rat and cell models of acute myocardial infarction

  • Immun Inflamm Dis. 2023 Feb;11(2):e775. doi: 10.1002/iid3.775.
Di Zhao 1,  Ketong Liu 2,  Jian Wang 1,  Haifeng Shao 1
Affiliations

Affiliations

  • 1 Department of Cardiology I, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang, China.
  • 2 Department of Cardiology, The Fifth Affiliated Hospital (Zhuhai) of Zunyi Medical University, Zhuhai, Guangdong, China.
PMID: 36840496 DOI: 10.1002/iid3.775
Abstract

Introduction: This study aimed to investigate the role of syringin in improving heart function during myocardial ischemia/reperfusion (I/R) and to determine whether the Sirtuin 1/Peroxisome Proliferator-activated Receptor gamma coactivator 1 alpha (SIRT1/PGC-1α) pathway contributes to this cardioprotective effect in vivo and in vitro.

Methods: H9c2 cells were incubated with H2 O2 for 12 h. The effect of syringin was assessed by measuring cell viability; the apoptotic rate; Keap1/NRF2/HO-1 activation; and the levels of proinflammatory cytokines, oxidative products, and antioxidative enzymes. In addition, SIRT1 was silenced via short hairpin RNA (shRNA)-SIRT1 transfection to evaluate its involvement in syringin-mediated protection. Syringin rescued cells from H2 O2 -induced reductions in viability, antioxidative Enzyme levels, and NRF2/HO-1 activation; likewise, syringin inhibited Apoptosis, inflammation, and oxidative stress. We also created a rat model of I/R by ligating the left anterior descending coronary artery for 30 min, followed by reperfusion for 12 min. Syringin was then intraperitoneally injected, and the effect on infarct size and cardiac function was examined after 7 days. NRF2/HO-1 activity and the levels of myocardial proinflammatory cytokines, oxidative products, and antioxidative enzymes were measured.

Results: In comparison to the untreated I/R group, the syringin treatment group exhibited improved cardiac function and reduced cardiac lesion and infarct size. Syringin administration also markedly reduced the levels of proinflammatory cytokines and Reactive Oxygen Species and promoted antioxidative Enzyme expression and NRF2/HO-1 pathway activation.

Conclusions: Syringin may serve a protective role in animal and cell models of I/R by improving cardiac function, inhibiting the inflammatory response, and activating the antioxidative response.

Keywords

SIRT1; inflammation; myocardial ischemia/reperfusion; oxidative stress; syringing.

Figures
Products