2. Academic Validation
  3. LncRNA HOXB-AS4 promotes proliferation and migration of colorectal cancer via the miR-140-5p/hdac7 axis

LncRNA HOXB-AS4 promotes proliferation and migration of colorectal cancer via the miR-140-5p/hdac7 axis

  • Biotechnol Genet Eng Rev. 2023 Mar 23;1-19. doi: 10.1080/02648725.2023.2193465.
Qican Deng 1,  Jianguo Yang 1,  Yajun Chen 2,  Zhenzhou Chen 2,  Juncai Li 1,  Zhongxue Fu 1
Affiliations

Affiliations

  • 1 Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
  • 2 Department of General Surgery, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.
PMID: 36951606 DOI: 10.1080/02648725.2023.2193465
Abstract

Long noncoding RNAs (lncRNA) have a critical role in colorectal Cancer (CRC) development and progression. However, the role of the lncRNA HOXB-AS4 in CRC remains unclear. In this study, we found that HOXB-AS4 was markedly upregulated in tumor tissues compared to precancerous tissues. Loss-of-function assays in HT29 and SW480 cells confirmed that knockdown of HOXB-AS4 inhibited proliferation, migration, and promoted Apoptosis. In addition, HOXB-AS4 was shown to regulate histone deacetylase 7 (HDAC7) expression by acting as a molecular Sponge to bind to and adsorb miR-140-5p. These findings were confirmed by the dual-luciferase reporter assay. Functional recovery experiments further demonstrated the crucial role of the HOXB-AS4/miR-140-5p/HDAC7 axis in modulating the malignant phenotype of CRC cells. Collectively, our data suggested that HOXB-AS4 regulated the malignant tumor aggression of HT29 and SW480 cells through the miR-140-5p/HDAC7 axis and PI3K/Akt signaling pathway. Our study provides novel insights into the mechanism of action of HOXB-AS4 in CRC and highlights its potential use as a targeted therapy.

Keywords

HDAC7; HOXB-AS4; MiR-140-5p; colorectal cancer.

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