2. Academic Validation
  3. Hydrogen Attenuates Inflammation by Inducing Early M2 Macrophage Polarization in Skin Wound Healing

Hydrogen Attenuates Inflammation by Inducing Early M2 Macrophage Polarization in Skin Wound Healing

  • Pharmaceuticals (Basel). 2023 Jun 15;16(6):885. doi: 10.3390/ph16060885.
Pengxiang Zhao 1 2 3,  Zisong Cai 1 2 3,  Xujuan Zhang 1 2 3,  Mengyu Liu 1 2 3,  Fei Xie 1 2 3,  Ziyi Liu 1 2 3,  Shidong Lu 1 2 3,  Xuemei Ma 1 2 3
Affiliations

Affiliations

  • 1 Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China.
  • 2 Beijing Molecular Hydrogen Research Center, Beijing 100124, China.
  • 3 Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing 100124, China.
PMID: 37375833 DOI: 10.3390/ph16060885
Abstract

The heterogeneous and highly plastic cell populations of macrophages are important mediators of cellular responses during all stages of wound healing, especially in the inflammatory stage. Molecular hydrogen (H2), which has potent antioxidant and anti-inflammatory effects, has been shown to promote M2 polarization in injury and disease. However, more in vivo time series studies of the role of M1-to-M2 polarization in wound healing are needed. In the current study, we performed time series experiments on a dorsal full-thickness skin defect mouse model in the inflammatory stage to examine the effects of H2 inhalation. Our results revealed that H2 could promote very early M1-to-M2 polarization (on days 2-3 post wounding, 2-3 days earlier than in conventional wound healing), without disturbing the functions of the M1 phenotype. Time series analysis of the transcriptome, blood cell counts, and multiple cytokines further indicated that peripheral blood monocytes were a source of H2-induced M2 macrophages and that the functions of H2 in macrophage polarization were not only dependent on its antioxidant effects. Therefore, we believe that H2 could reduce inflammation in wound care by shifting early macrophage polarization in clinical settings.

Keywords

M2 macrophage polarization; anti-ROS independent; in vivo time series study; inflammation stage; molecular hydrogen; wound healing.

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