2. Academic Validation
  3. IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4+T cells

IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4+T cells

  • iScience. 2023 Jul 25;26(8):107464. doi: 10.1016/j.isci.2023.107464.
Yanyu Zhang 1,  Song Gao 2,  Shifei Yao 1,  Danlin Weng 1,  Yan Wang 1,  Qi Huang 1,  Xuemei Zhang 1,  Hong Wang 1,  Wenchun Xu 1
Affiliations

Affiliations

  • 1 Key Laboratory of Laboratory Medical Diagnostics Designated by the Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing, China.
  • 2 Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical University, School of Laboratory Medicine, Zunyi Medical University, Zunyi, Guizhou, China.
PMID: 37588169 DOI: 10.1016/j.isci.2023.107464
Abstract

Vaccination is an effective means of preventing pneumococcal disease and SPY1 is a live attenuated pneumococcal vaccine we obtained earlier. We found IL-27 and its specific receptor (WSX-1) were increased in SPY1 vaccinated mice. Bacterial clearance and survival rates were decreased in SPY1 vaccinated IL-27Rα-/- mice. The vaccine-induced Th17 cell response and IgA secretion were also suppressed in IL-27Rα-/- mice. STAT3 and NF-κB signaling and expression of the Th17 cell polarization-related cytokines were also decreased in IL-27Rα-/- bone-marrow-derived dendritic cells(BMDC) stimulated with inactivated SPY1. The numbers of memory CD4+T cells were also decreased in SPY1 vaccinated IL-27Rα-/- mice. These results suggested that IL-27 plays a protective role in SPY1 vaccine by promoting Th17 polarization through STAT3 and NF-κB signaling pathways and memory CD4+T cells production in the SPY1 vaccine. In addition, we found that the immune protection of SPY1 vaccine was independent of aerobic glycolysis.

Keywords

Immune response; Immunology; Molecular biology; Virology.

Figures
Products