2. Academic Validation
  3. Steroidal Alkaloids from the Roots of Veratrum mengtzeanum Loes. with Their Anti-Inflammatory Activities

Steroidal Alkaloids from the Roots of Veratrum mengtzeanum Loes. with Their Anti-Inflammatory Activities

  • Molecules. 2023 Oct 16;28(20):7116. doi: 10.3390/molecules28207116.
Wenjuan Yuan 1 2,  Jinrong Ma 2 3,  Xinlan Liu 2 3,  Chengting Zi 1 2,  Yongkai Xi 1 2,  Xiaojing Shen 1,  Guodong Li 4,  Jun Sheng 2,  Xuanjun Wang 2
Affiliations

Affiliations

  • 1 College of Science, Yunnan Agricultural University, Kunming 650201, China.
  • 2 Key Laboratory of Pu'er Tea, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China.
  • 3 College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
  • 4 College of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China.
PMID: 37894597 DOI: 10.3390/molecules28207116
Abstract

The phytochemical investigation of Veratrum mengtzeanum Loes. roots resulted in the isolation and characterization of two novel, namely Mengtzeanines A (1), Mengtzeanines B (2), and eight known steroidal Alkaloids (3-10). Their structural properties were assessed though extensive spectroscopic techniques. All constituents 1-10 were analyzed for suppression of NO formation in LPS-induced RAW264.7 macrophages. Among them, constituent 6 (Verazine) showed inhibition against LPS-induced NO production (IC50 = 20.41 μM). Additionally, compound 6 could inhibit the secretion of IL1β, IL6, and TNFα, and downregulate the productions of iNOS and COX2 in LPS-induced RAW264.7 macrophages. Further experiments revealed that 6 exhibited a potent anti-inflammatory level in LPS-stimulated RAW264.7 macrophages via inhibiting NF-κB, and triggering of Keap1/Nrf2/HO-1 axis, implying that compound 6 may be a promising candidate for treating inflammatory disorders.

Keywords

Veratrum mengtzeanum Loes.; anti-inflammatory activity; mengtzeanines A; mengtzeanines B; steroidal alkaloids.

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