2. Academic Validation
  3. Pharmacokinetics and tissue distribution of key sesquiterpene glycosides in Dendrobium nobile analyzed by UHPLC-Q-Trap-MS/MS

Pharmacokinetics and tissue distribution of key sesquiterpene glycosides in Dendrobium nobile analyzed by UHPLC-Q-Trap-MS/MS

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2025 Jan 1:1250:124386. doi: 10.1016/j.jchromb.2024.124386.
Xingdong Wu 1,  Chunxue Gao 1,  Ya Huang 2,  Lin Qin 1,  Zhou Yang 3,  Di Wu 1,  Ya Wang 1,  Qianru Zhang 1,  Daopeng Tan 1,  Yongxia Zhao 1,  Jiajia Wu 4,  Shanyong Yi 5,  Yanliu Lu 6,  Yuqi He 7
Affiliations

Affiliations

  • 1 Guizhou Engineering Research Center of Industrial Key-technology for Dendrobium Nobile, Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 551800, China.
  • 2 Jinsha County Chinese Medicine Hospital, Bijie, Guizhou 550016, China.
  • 3 Guizhou Standard Pharmaceutical Health Co., Ltd, Zunyi 563000, China.
  • 4 Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
  • 5 Department of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, Anhui, China.
  • 6 Guizhou Engineering Research Center of Industrial Key-technology for Dendrobium Nobile, Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 551800, China. Electronic address: [email protected].
  • 7 Guizhou Engineering Research Center of Industrial Key-technology for Dendrobium Nobile, Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 551800, China. Electronic address: [email protected].
PMID: 39603010 DOI: 10.1016/j.jchromb.2024.124386
Abstract

Dendrobium nobile (D. nobile), a traditional herb known for its immunomodulatory and neuroprotective properties, contains characteristic Alkaloids and sesquiterpene glycosides. While Alkaloids have been extensively studied, research on sesquiterpene glycosides remains limited. This study established and validated a UHPLC-Q-Trap-MS/MS method for detecting six sesquiterpene glycosides in D. nobile, applying it to pharmacokinetic and tissue distribution studies in rats following oral administration of the D. nobile aqueous extract. Plasma and tissue samples were prepared using methanol for protein precipitation and separated on a Waters Acquity UPLC BEH C18 column. Quantification was performed using multiple reaction monitoring (MRM) in negative electrospray ionization (ESI) mode. Method validation demonstrated specificity, selectivity, precision, accuracy, stability, matrix effects, and recovery rates meeting the criteria for in vivo drug analysis. Pharmacokinetic results indicated that dendronobiloside A, dendronobiloside C, and dendronobiloside D were rapidly absorbed with low plasma concentrations and quick elimination. In contrast, dendronobiloside E, dendroside G, and dendromoniliside D were rapidly absorbed with higher plasma concentrations but also eliminated quickly. Tissue distribution studies revealed that dendronobiloside A, C, and D were detectable in the heart, liver, spleen, lungs, kidneys, stomach, large intestine, small intestine, thymus, and pancreas, but almost undetectable in the brain. And dendronobiloside E, dendroside G, and dendromoniliside D were detectable in all tissues. Overall, the six sesquiterpene glycosides reached various tissues within 2 h of administration, with distribution levels ranked as follows: small intestine > stomach > large intestine > pancreas > lungs > kidneys > liver > heart > thymus > spleen > brain. These findings provide insights into the immunomodulatory mechanisms of D. nobile sesquiterpene glycosides and inform clinical dosing considerations.

Keywords

Dendrobium nobile; Pharmacokinetics; Sesquiterpene glycosides; Tissue distribution; UHPLC-Q-Trap-MS/MS.

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