2. Academic Validation
  3. Activity of 6-methoxydihydrosanguinarine from Hylomecon japonica against wild-type and fluconazole-resistant Candida albicans biofilms

Activity of 6-methoxydihydrosanguinarine from Hylomecon japonica against wild-type and fluconazole-resistant Candida albicans biofilms

  • J Asian Nat Prod Res. 2025 Jul 28:1-12. doi: 10.1080/10286020.2025.2532041.
Ying Liu 1,  Miao Wang 1,  Guang-Xiong Zhou 1 2
Affiliations

Affiliations

  • 1 Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 511443, China.
  • 2 Health and Medicine Science College, Guangzhou Huashang College, Guangzhou 511300, China.
PMID: 40720099 DOI: 10.1080/10286020.2025.2532041
Abstract

The threat posed by Microorganisms to food safety in development necessitates the discovery of new antimicrobial agents. Here, we unravel the Antifungal activity of 6-methoxydihydrosanguinarine (6-ME) derived from Hylomecon japonica. 6-ME disrupted the biofilm formation of Candida albicans (C. albicans). 6-ME could cause the blockage of filamentation and classical cyclic adenosine monophosphate (cAMP) signaling pathway is involved in this process. 6-ME downregulated the expression of EFG1, CDC35, RAS1, and TPK2, all of which are critical components of the cAMP pathway. Furthermore, 6-ME changed the membrane permeability of C. albicans and caused Reactive Oxygen Species accumulation, leading to cell death.

Keywords

6-Methoxydihydrosanguinarine; Candida albicans; antifungal; biofilms; cAMP pathway.

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