2. Academic Validation
  3. Development of the FGFR4-ADC bearing the ferroptosis inducer sulfasalazine for hepatocellular carcinoma

Development of the FGFR4-ADC bearing the ferroptosis inducer sulfasalazine for hepatocellular carcinoma

  • iScience. 2025 Oct 6;28(11):113718. doi: 10.1016/j.isci.2025.113718.
Ying Zhang 1 2 3,  Xiaohong Cui 4,  Jing Du 5,  Ge Cui 6,  Yuhang Ling 1 2 3,  Chengwu Tang 1 2 7 3
Affiliations

Affiliations

  • 1 Central Laboratory, First Affiliated Hospital of Huzhou University, Huzhou 313000, China.
  • 2 Huzhou Key Laboratory of Translational Medicine, First People's Hospital of Huzhou, Huzhou 313000, China.
  • 3 Key Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, First People's Hospital of Huzhou, Huzhou 313000, China.
  • 4 Psychiatry Department, Shanxi Bethune Hospital, Taiyuan 030032, China.
  • 5 Key Laboratory of Aerospace Medicine of Ministry of Education, School of Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China.
  • 6 Department of Pathology, First Affiliated Hospital of Huzhou University, Huzhou 313000, China.
  • 7 Department of Hepatopancreatobiliary Surgery, First Affiliated Hospital of Huzhou University, Huzhou 313000, China.
PMID: 41210985 DOI: 10.1016/j.isci.2025.113718
Abstract

Hepatocellular carcinoma (HCC), the most prevalent liver Cancer, accounts for ∼90% of all cases. Despite antibody-drug conjugates (ADCs) showing significant efficacy in treating various hematologic malignancies and solid tumors, their application in HCC treatment is unimpressive. In this study, we identified that Fibroblast Growth Factor receptor 4 (FGFR4) is highly expressed in HCC patient tumors and its expression correlates with overall survival rates, suggesting that FGFR4 could be an excellent therapeutic target for HCC. We subsequently developed an innovative anti-FGFR4 antibody exhibiting robust binding and endocytosis activities within HCC cells. Utilizing a GGFG linker coupled with the Ferroptosis inducer sulfasalazine, we produced an FGFR4-ADC compound. This ADC specifically induced Ferroptosis in FGFR4-expressing HCC cells and demonstrated FGFR4 expression-dependent anti-tumor activity in xenograft models. The preclinical pharmacokinetics and safety profile of our developed FGFR4-ADC suggest it may represent a pioneering ADC molecule offering a strategy for treating patients with tumor-associated FGFR4 expression.

Keywords

Biotechnology; Cancer; Therapeutics.

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