2. Academic Validation
  3. Development of retinoid nuclear receptor pathway antagonists through targeting aldehyde dehydrogenase 1A3

Development of retinoid nuclear receptor pathway antagonists through targeting aldehyde dehydrogenase 1A3

  • iScience. 2025 Oct 3;28(11):113675. doi: 10.1016/j.isci.2025.113675.
Mark Esposito 1 2 3,  Cao Fang 1 3,  Yong Wei 1 3,  Alfonso Pozzan 4,  Claudia Beato 4,  Xiaoyang Su 5,  Josiah E Hutton 3rd 1,  Tavis Reed 1,  Xiang Hang 1 3,  Enrico D Perini 6,  Wen Wang 6,  Xiaobing Cheng 6,  Yan Pan 6,  Jianshi Yu 7,  Maureen Kane 7,  Malini Manoharan 8,  John Proudfoot 9,  Ileana M Cristea 1,  Yibin Kang 1 3
Affiliations

Affiliations

  • 1 Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
  • 2 Kayothera Inc, Seattle, WA 98102, USA.
  • 3 Ludwig Institute for Cancer Research Princeton Branch, Princeton, NJ 08544, USA.
  • 4 Aptuit (Verona) Srl, an Evotec Company, Verona, Italy.
  • 5 Rutgers University Robert Wood Johnson Medical School, New Brunswick, NJ 08904, USA.
  • 6 WuXi AppTec, Shanghai, China.
  • 7 Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD 21201, USA.
  • 8 DeepKnomics Labs, Bangalore, India.
  • 9 DiscoveryBytes LLC, Newtown, CT, USA.
PMID: 41210994 DOI: 10.1016/j.isci.2025.113675
Abstract

Aldehyde dehydrogenase 1a3 (ALDH1A3) activity is recognized as a pathogenic trait in cardiometabolic diseases and Cancer, though the mechanisms by which ALDH1A3 promotes disease are unclear and effective therapeutic inhibitors of ALDH1A3 are lacking. Whereas the function of the ALDH1A Enzymes in development is the conversion of retinaldehyde into all-trans retinoic acid (atRA) to activate retinoid nuclear receptor signaling, this pathway is paradoxically hypothesized as a cell-intrinsic tumor suppressor pathway. We resolve this paradox by showing that while ALDH1A3 is overexpressed across diverse cancers, ALDH1A3-expressing tumor cells lose sensitivity to retinoid signaling. Instead, atRA produced by ALDH1A3 acts in a paracrine fashion to activate retinoid nuclear receptor signaling in immune cells to suppress anti-tumor immunity. To inhibit ALDH1A3, we developed a hybrid in silico and high-throughput screening approach followed by medicinal optimization to identify first-in-class, oral and safe antagonists of ALDH1A3 with potent anti-tumor immunotherapeutic activity and an optimized drug development profile.

Keywords

Cancer; Cell biology.

Figures
Products