2. Academic Validation
  3. Study on the mechanism of hUCMSCs-derived 3D exosome combined with HAMA microneedles in the treatment of deep second-degree burns

Study on the mechanism of hUCMSCs-derived 3D exosome combined with HAMA microneedles in the treatment of deep second-degree burns

  • Bioact Mater. 2025 Oct 29:56:468-482. doi: 10.1016/j.bioactmat.2025.09.031.
Mariya M J Elakkawi 1,  Yingjie Zhu 2,  Yang Xiong 1,  Changhong Li 1,  Hantao Li 1,  Jianwei Chen 3,  Tao Xu 3,  Xiangdong Qi 1
Affiliations

Affiliations

  • 1 Department of Plastic and Aesthetic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • 2 Department of Hemangioma and Vascular Malformation, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
  • 3 Center for Bio-intelligent Manufacturing and Living Matter Bioprinting, Research Institute of Tsinghua University in Shenzhen, Tsinghua University, Shenzhen, China.
PMID: 41234297 DOI: 10.1016/j.bioactmat.2025.09.031
Abstract

Burn wound healing remains challenging due to uncontrolled inflammation and poor angiogenesis. We developed hyaluronic acid methacryloyl microneedles (EXO@HAMA) loaded with 3D-cultured mesenchymal stem cell (MSC) exosomes to overcome limitations of growth factor therapies and sudden release of exosomes. 3D-exosomes potently enhanced fibroblast proliferation and endothelial tube formation. In rat deep second-degree burns, EXO@HAMA reduced inflammation and accelerated healing through CTSB/TGF-β and Wnt/β-catenin axis modulation, significantly improving Collagen deposition and angiogenesis. Transcriptomics revealed activation of lipid metabolism and extracellular matrix (ECM) remodeling. This work demonstrates 3D-exosome/HAMA synergy in resolving inflammation-angiogenesis imbalance, offering a clinically promising strategy for burn regeneration.

Keywords

Burn; Exosome; HAMA; Microneedle.

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