2. Academic Validation
  3. Evolution of Multivalent Aptamer Corona for High-Throughput Multiplexed Detection of Multiple Cancers

Evolution of Multivalent Aptamer Corona for High-Throughput Multiplexed Detection of Multiple Cancers

  • Adv Sci (Weinh). 2025 Nov 25:e14976. doi: 10.1002/advs.202514976.
Mengjie Wang 1 2,  Baichuan Jin 2,  Xin Dai 2,  Haozhe Huang 2,  Qiqi Liu 2 3,  Jianqing Zhu 2,  Haixing Ju 2,  Qixun Chen 2,  Yongmei Song 4,  Weihong Tan 1 2,  Yuan Liu 2
Affiliations

Affiliations

  • 1 Institute of Department, Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China.
  • 2 Institute of Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China.
  • 3 Institute of Cancer Centre and Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, 999078, China.
  • 4 State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.
PMID: 41290548 DOI: 10.1002/advs.202514976
Abstract

Clinical in vitro diagnosis is essential in early diagnosis and prognostic assessment, yet antibody-based assays face limitations in multiplexity and scalability. To address the growing demand for modern molecular diagnostics, a multivalent aptamer corona platform for high-throughput is introduced and multiplexed multi-cancer diagnosis. With clinical serum samples, ovarian cancer-, lung cancer-, and colorectal cancer-based aptamer coronas are obtained through several rounds of alternating positive and negative systematic evolution of ligands by exponential enrichment (SELEX). Leveraging a de novo ProteoFish-SELEX strategy, which integrates nanoparticle-protein corona technology, a multivalent aptamer corona is evolved. Proteomic profiling of protein coronas revealed cancer-associated protein signatures, while aptomic profiling of aptamer coronas identified high-affinity cancer-specific Aptamers. With these Aptamers, high diagnostic accuracy is demonstrated in multiplexed multi-cancer detection using clinical cohorts. Multivalent aptamer corona's outstanding diagnostic performance, multiplexed detection capability, and sequencing-enabled high-throughput potential position it as a promising versatile tool for novel biomarker discovery and a transformative advancement in precision oncology.

Keywords

ProteoFish‐SELEX; aptomics; multiplexed cancer diagnosis; multivalent aptamer corona; proteomics.

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