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Fibronectin Protein, Human

9 Cited Publications
Customer Review

Based on 9 publication(s) in Google Scholar

Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. Fibronectin Protein, Human is the recombinant human-derived Fibronectin protein, expressed by E. coli, with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Fibronectin Protein, Human Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. Fibronectin Protein, Human is the recombinant human-derived Fibronectin protein, expressed by E. coli, with tag free.

Background

Fibronectin, a versatile glycoprotein, plays a crucial role in cellular interactions by binding to various compounds such as collagen, fibrin, heparin, DNA, and actin. Its involvement in cell adhesion, motility, opsonization, wound healing, and maintenance of cell shape underscores its significance in fundamental cellular processes. Fibronectin also contributes to osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization, and regulates the deposition of type I collagen by osteoblasts. Acting as a ligand for the LILRB4 receptor, Fibronectin inhibits FCGR1A/CD64-mediated monocyte activation. Moreover, Fibronectin's ability to induce fibril formation, as observed in the fibronectin polymer named superfibronectin, imparts enhanced adhesive properties. Notably, both anastellin and superfibronectin exhibit inhibitory effects on tumor growth, angiogenesis, and metastasis, with anastellin activating p38 MAPK and inhibiting lysophospholipid signaling. The multifaceted functions of Fibronectin underscore its pivotal role in cellular dynamics and its potential as a target for therapeutic interventions in various pathological conditions.

In Vitro

Fibronectin protein (Human) (2-40 nM, 24-100 h) dose-dependently promotes the growth of mouse embryonic cells lacking endogenous fibronectin[11].
Fibronectin protein (Human) has high binding affinity for the human LILRB4 receptor and its direct homolog gp49B receptor in mice, with KD values of 421 nM and 50.1 nM, respectively[12].

In Vivo

FN30-Fc is a recombinant protein formed by fusing the N-terminal 30 kDa fragment of human fibronectin (FN30) with mouse IgG2a Fc. FN30-Fc (200 μg, intraperitoneally injected into 10-week-old mice, a total of 2 doses with a 2-week interval) shows efficacy in improving autoimmune nephritis in the BXSB/Yaa lupus (SLE) mouse model[12].

Verified Bioactivity

1.Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is ≤ 385.7 ng/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is ≤182.9 ng/mL, corresponding to a specific activity is ≥5.467×103 units/mg.

Results
  • Experimental Validation Results for Fibronectin Protein, Human
    Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×103 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P02751-1 (P1270-S1546&A1721-T2016)

  • Gene ID
    2335  [NCBI]
  • Molecular Construction
    • N-term
    • Fibronectin (P1270-S1546 & A1721-T2016)
      Accession # P02751-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NovoNectin; Fibronectin; FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1

  • AA Sequence

    PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPS&AIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST

  • Molecular Weight

    Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for Fibronectin Protein, Human
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 12.5 mM sodium citrate, 1.25% sucrose, pH 5.5.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.8, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<0.01 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Patten J, Wang K. Fibronectin in development and wound healing. Adv Drug Deliv Rev. 2021 Mar;170:353-368. [Content Brief]

[2]. Lenselink EA. Role of fibronectin in normal wound healing. Int Wound J. 2015 Jun;12(3):313-6. doi: 10.1111/iwj.12109. Epub 2013 Jun 7. [Content Brief]

[3]. Bielefeld K A, et al. Fibronectin and β-catenin act in a regulatory loop in dermal fibroblasts to modulate cutaneous healing[J]. Journal of Biological Chemistry, 2011, 286(31): 27687-27697. [Content Brief]

[4]. Shi F, et al. MT1-MMP regulates the turnover and endocytosis of extracellular matrix fibronectin[J]. Journal of cell science, 2011, 124(23): 4039-4050. [Content Brief]

[5]. Lefcort F, et al. Regulation of expression of fibronectin and its receptor, α5β1, during development and regeneration of peripheral nerve[J]. Development, 1992, 116(3): 767-782.

[6]. Clark RA, et al. Blood vessel fibronectin increases in conjunction with endothelial cell proliferation and capillary ingrowth during wound healing. J Invest Dermatol. 1982 Nov;79(5):269-76. [Content Brief]

[7]. Oyama F, et al. Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors. J Biol Chem. 1989 Jun 25;264(18):10331-4. [Content Brief]

[8]. Yi M, Ruoslahti E. A fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis. Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):620-4. [Content Brief]

[9]. Quirós J, et al. Beneficial effect of fibronectin administration on chronic nephritis in rats. Arthritis Rheum. 1990 May;33(5):685-92. [Content Brief]

[10]. Su MT, et al. Blockade of checkpoint ILT3/LILRB4/gp49B binding to fibronectin ameliorates autoimmune disease in BXSB/Yaa mice. Int Immunol. 2021 Jul 23;33(8):447-458. [Content Brief]

[11]. Sottile J, et al. Fibronectin matrix assembly enhances adhesion-dependent cell growth. J Cell Sci. 1998 Oct;111 ( Pt 19):2933-43. [Content Brief]

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Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×103 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×103 units/mg.

  • 1/16

    Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Customer Validation
     Relative mRNA expression of CD206 in macrophages treated with FN1 alone (20 μg/mL), FN1 + CWHM-12 (pan-αV integrin inhibitor), or FN1 + M200 (α5β1 integrin-blocking antibody).

  • 1/16

    Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Customer Validation
     The expression of M2 markers MRC1 and ARG1 in macrophages was analyzed by Western blotting, with or without Fibronectin Protein, Human (FN1: 20 μg/mL) and M200 treatment, respectively.

  • 1/16

    Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Customer Validation
     Representative flow cytometry images and quantitative analysis results of CD163+ cell populations in THP-1-derived and primary macrophages treated or untreated with Fibronectin Protein, Human (FN1: 20 μg/mL) and M200.

  • 1/16

    Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Customer Validation
     The final tumor weight showed the antitumor effect of silengilide and its partial reversal by Fibronectin Protein, Human (FN1: 10 μg/kg).

  • 1/16

    Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Customer Validation
     Fibronectin Protein, Human (FN1: 10 μg/kg). Multiplex immunofluorescence images of tumor sections show the distribution of F4/80+ macrophages (green) expressing CD86 or CD206 (red) in each treatment group. Cell nuclei were counterstained with DAPI (blue).

  • Fibronectin Protein, Human Product Image
    1/16
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Experimental Validation Results for Fibronectin Protein, Human
  • Fibronectin Protein, Human Product Image

  • Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Relative mRNA expression of CD206 in macrophages treated with FN1 alone (20 μg/mL), FN1 + CWHM-12 (pan-αV integrin inhibitor), or FN1 + M200 (α5β1 integrin-blocking antibody).

  • Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    The expression of M2 markers MRC1 and ARG1 in macrophages was analyzed by Western blotting, with or without Fibronectin Protein, Human (FN1: 20 μg/mL) and M200 treatment, respectively.

  • Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Representative flow cytometry images and quantitative analysis results of CD163+ cell populations in THP-1-derived and primary macrophages treated or untreated with Fibronectin Protein, Human (FN1: 20 μg/mL) and M200.

  • Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    The final tumor weight showed the antitumor effect of silengilide and its partial reversal by Fibronectin Protein, Human (FN1: 10 μg/kg).

  • Fibronectin Protein, Human purchased from MedChemExpress. Usage Cited in: Oncogene. 2025 Jul;44(28):2396-2412. [Abstract]

    Fibronectin Protein, Human (FN1: 10 μg/kg). Multiplex immunofluorescence images of tumor sections show the distribution of F4/80+ macrophages (green) expressing CD86 or CD206 (red) in each treatment group. Cell nuclei were counterstained with DAPI (blue).