Protein S/PROS1 Protein, Human (HEK293, His)

Protein S (PS) is a vitamin K-dependent glycoprotein which plays an important role in the regulation of blood coagulation. Protein S/PROS1 Protein, Human (HEK293, His) is the recombinant human-derived protein S/PROS1 protein, expressed by HEK293 , with C-His labeled tag.

Protein S (PS) is a vitamin K-dependent glycoprotein that acts as a natural anticoagulant. It is synthesized by hepatocytes, endothelial cells, megakaryocytes, testis Leydig cells and in brain. Protein S and APC form the activated protein C (APC) complex, which is responsible for the proteolytic deactivation of activated factors (F) V (Va) and VIII (VIIIa). Protein S is also identified as a cofactor of tissue factor pathway inhibitor (TFPI), which stimulates inhibition of the FXa by TFPI through the APC-independent mechanism^[1].

Measured by its binding ability in a functional ELISA.When Recombinant Human Tyro3/Dtk (HY-P78657) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Protein S/PROS1 binds with an ED₅₀ of 0.04-0.10 μg/mL.

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  Measured by its binding ability in a functional ELISA.When Recombinant Human Tyro3/Dtk (HY-P78657) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Protein S/PROS1 binds with an ED₅₀ of 0.04361 μg/mL.

Human

HEK293

C-10*His

P07225 (N25-S676)

5627  [NCBI]

Full Length of Mature Protein

NFLSKQQASQVLVRKRRANSLLEETKQGNLERECIEELCNKEEAREVFENDPETDYFYPKYLVCLRSFQTGLFTAARQSTNAYPDLRSCVNAIPDQCSPLPCNEDGYMSCKDGKASFTCTCKPGWQGEKCEFDINECKDPSNINGGCSQICDNTPGSYHCSCKNGFVMLSNKKDCKDVDECSLKPSICGTAVCKNIPGDFECECPEGYRYNLKSKSCEDIDECSENMCAQLCVNYPGGYTCYCDGKKGFKLAQDQKSCEVVSVCLPLNLDTKYELLYLAEQFAGVVLYLKFRLPEISRFSAEFDFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS

Approximately 78-98 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wypasek E, et al. Venous thromboembolism associated with protein S deficiency due to Arg451* mutation in PROS1 gene: a case report and a literature review. J Genet. 2017 Dec;96(6):1047-1051.  [Content Brief]