TYRO3/DTK Protein, Human (Q261H, HEK293, Fc)

The TYRO3/DTK protein is a tyrosine kinase receptor. TYRO3 transduces signals from the extracellular matrix by activating the PI3K/AKT/ NF-kappa-B pathway. TYRO3 plays an important role in inhibiting Toll-like receptor (TLRs) mediated innate immune response by activating STAT1. TYRO3 has the function of promoting tumor cell survival and/or proliferation, metastasis and chemotherapy resistance. TYRO3/DTK Protein, Human (Q261H, HEK293, Fc) is the recombinant human-derived TYRO3/DTK protein, expressed by HEK293 , with C-hFc labeled tag.

TYRO3/DTK is a receptor tyrosine kinase that transduces signals from the extracellular matrix to the cytoplasm by binding to several ligands, including TULP1 or GAS6. Regulates many physiological processes, including cell survival, migration, and differentiation. The binding of ligands on the cell surface induced dimerization and autophosphorylation of TYRO3 in the intracellular region, providing docking sites for downstream signaling molecules. After the ligand is activated, it interacts with PIK3R1 to enhance PI3 kinase activity. Activation of AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of NF-kappa-B regulatory genes. TYRO3 signaling plays a role in a variety of processes, such as neuronal protection from excitotoxic damage, platelet aggregation, and cytoskeletal recombination. By activating STAT1, which plays an important role in Toll-like receptor (TLRs) mediated innate immune response, STAT1 selectively induces the production of inhibitors of cytokine signaling pathways SOCS1 and SOCS3. TYRO3 is overexpressed in many types of cancer and has the function of promoting tumor cell survival and/or proliferation, metastasis, and chemotherapy resistance^[1][2].

1.This product does not contain protein kinase domain.
2.Immobilized TYRO3 at 2 μg/mL (100 μL/well) can bind Biotinylated GAS6 protein. The ED₅₀ for this effect is 161.5 ng/mL, corresponding to a specific activity is 6.19×10^3 Unit/mg.

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  Immobilized TYRO3 at 2 μg/mL (100 μL/well) can bind Biotinylated GAS6 protein. The ED₅₀ for this effect is 161.5 ng/mL, corresponding to a specific activity is 6.19×10³ Unit/mg.

Human

HEK293

C-hFc

Q06418/AAH51756.1 (A41-S428, Q261H)

7301  [NCBI]

Extracellular Domain

AGLKLMGAPVKLTVSQGQPVKLNCSVEGMEEPDIQWVKDGAVVQNLDQLYIPVSEQHWIGFLSLKSVERSDAGRYWCQVEDGGETEISQPVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIVWWRGTTKIGGPAPSPSVLNVTGVTQSTMFSCEAHNLKGLASSRTATVHLQALPAAPFNITVTKLSSSNASVAWMPGADGRALLQSCTVHVTQAPGGWEVLAVVVPVPPFTCLLRDLVPATNYSLRVRCANALGPSPYADWVPFQTKGLAPASAPQNLHAIRTDSGLILEWEEVIPEAPLEGPLGPYKLSWVQDNGTQDELTVEGTRANLTGWDPQKDLIVRVCVSNAVGCGPWSQPLVVSSHDRAGQQGPPHSRTS

Approximately 85-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCL, 100 mM arginine, 100 mM Glycine, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.