2. Academic Validation
  3. An Automated Organotypic SCN Culture System Revealing Novel Insights into VIP Regulation of Circadian Rhythm

An Automated Organotypic SCN Culture System Revealing Novel Insights into VIP Regulation of Circadian Rhythm

  • Adv Sci (Weinh). 2026 Feb;13(10):e11069. doi: 10.1002/advs.202511069.
Kui Han 1,  Meimei Liao 2,  Jingpeng Zhang 1 3,  Ruoyu Zhong 4,  Long Mei 5,  Dapeng Ju 2,  Eric Erquan Zhang 5 6,  Yanyi Huang 1 7 8
Affiliations

Affiliations

  • 1 Biomedical Pioneering Innovation Center (BIOPIC), Peking-Tsinghua Center for Life Sciences, College of Chemistry, Peking University, Beijing, China.
  • 2 Department of Anesthesiology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
  • 3 School of Life Sciences, Peking University, Beijing, China.
  • 4 Integrated Science Program, Yuanpei College, Peking University, Beijing, China.
  • 5 National Institute of Biological Sciences, Beijing, China.
  • 6 Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China.
  • 7 College of Chemistry and Molecular Engineering, Beijing National Laboratory of Molecular Sciences, Peking University, Beijing, China.
  • 8 Shenzhen Bay Laboratory, Institute for Cell Analysis, Guangdong, China.
PMID: 41536242 DOI: 10.1002/advs.202511069
Abstract

In mammals, the suprachiasmatic nucleus (SCN) functions as the primary driver of circadian rhythm. Although individual SCN neurons exhibit cell-autonomous oscillations, intercellular communication-mediated by neural peptides such as vasoactive intestinal polypeptide (VIP)-synchronizes their rhythms. However, the precise molecular mechanism by which VIP facilitates SCN synchronization remains elusive. Here, we introduce an automated ex vivo culture system, termed 'brain-slice-in-a-chamber' (BaSIC), tailored for SCN slices, as well as dissociated cells culture. This apparatus automates medium exchange and ensures optimal control over temperature and humidity, ensuring a stable internal environment conducive to tissue culture. Furthermore, BaSIC enables real-time observation of tissue responses to diverse but programmed stimuli. Using BaSIC, we demonstrate that VIP pulsing rapidly resets the circadian rhythm by synchronizing both phase and amplitude through a swift reduction of Period 2 (PER2) protein. Mathematical modeling, coupled with experimental validation, further suggests that VIP promotes the rapid reduction of PER2. Our findings, facilitated by BaSIC, provide new insights into SCN neuron synchronization, paving the way for advanced studies in chronobiology, with potential therapeutic applications for circadian disorders.

Keywords

PER2 reduction; circadian rhythm; ex vivo culture; suprachiasmatic nucleus.

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