2. Academic Validation
  3. Nuclear prostaglandin E synthase 3 promotes hepatocellular carcinoma growth with immunosuppressive macrophage polarization via the SP1/TGF-β axis

Nuclear prostaglandin E synthase 3 promotes hepatocellular carcinoma growth with immunosuppressive macrophage polarization via the SP1/TGF-β axis

  • Mol Biomed. 2026 Mar 25;7(1):37. doi: 10.1186/s43556-026-00431-6.
Nianfei Wang 1 2 3,  Wei Chen 4,  Shumin Shen 3,  Jian Qi 1 2,  Shanghu Wang 1 2 5,  Rong Wang 6,  Ming Li 7,  Zixiang Chen 8,  Jiangming Chen 9,  Bo Hong 10 11 12,  Hongzhi Wang 13 14 15
Affiliations

Affiliations

  • 1 Hefei Cancer Hospital of CAS, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences (CAS), Hefei, Anhui, 230031, China.
  • 2 Science Island Branch, Graduate School of the University of Science and Technology of China, Hefei, Anhui, 230026, China.
  • 3 Department of Oncology, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China.
  • 4 Department of General Surgery, Hefei Affiliated Hospital of Anhui Medical University, Hefei, 230011, China.
  • 5 Department of Radiation Therapy, Anhui Chest Hospital, Hefei, 230026, China.
  • 6 Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, 230601, China.
  • 7 Department of Pathology, The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China.
  • 8 Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230601, China.
  • 9 Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230601, China. [email protected].
  • 10 Hefei Cancer Hospital of CAS, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences (CAS), Hefei, Anhui, 230031, China. [email protected].
  • 11 Science Island Branch, Graduate School of the University of Science and Technology of China, Hefei, Anhui, 230026, China. [email protected].
  • 12 School of Basic Medicine, Anhui Medical University, Hefei, Anhui, 230032, China. [email protected].
  • 13 Hefei Cancer Hospital of CAS, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences (CAS), Hefei, Anhui, 230031, China. [email protected].
  • 14 Science Island Branch, Graduate School of the University of Science and Technology of China, Hefei, Anhui, 230026, China. [email protected].
  • 15 School of Basic Medicine, Anhui Medical University, Hefei, Anhui, 230032, China. [email protected].
PMID: 41880053 DOI: 10.1186/s43556-026-00431-6
Abstract

Hepatocellular carcinoma (HCC) is characterized by the synchronization of tumor cell proliferation and an immunosuppressive microenvironment. Decoupling these interconnected processes represents a major therapeutic challenge. Although Prostaglandin E Synthase 3 (PTGES3) functions canonically as a cytoplasmic Heat Shock Protein 90 (HSP90) co-chaperone, its non-canonical nuclear role in orchestrating tumor-immune crosstalk remains undefined. Here, we identify PTGES3 as a dual-function regulator coupling tumor intrinsic growth with extrinsic immune remodeling. We report that PTGES3 is upregulated in HCC and serves as an independent prognostic factor for poor survival. Using an immunocompetent, diethylnitrosamine (DEN)-induced HCC mouse model, we demonstrate that hepatocyte-specific Ptges3 silencing significantly suppresses tumorigenesis. Single-cell RNA Sequencing (scRNA-seq) and histological analysis reveal that PTGES3 deficiency remodels the immune landscape, specifically by impairing tumor-associated macrophage (TAM) infiltration and M2 polarization. Mechanistically, we identified a specific G-rich motif on the Specificity Protein 1 (SP1) promoter bound by PTGES3 (confirmed via electrophoretic mobility shift assay [EMSA] and Cleavage Under Targets and Tagmentation [CUT&Tag]), which drives SP1-mediated Transforming Growth Factor-β (TGF-β) secretion. This axis appears to exert dual oncogenic effects: triggering paracrine M2 macrophage polarization to foster immunosuppression, whilst simultaneously fueling an autocrine TGF-β/TGFBR/PI3K/Akt/mTOR signaling loop to sustain tumor proliferation. Our findings define a non-canonical nuclear function for PTGES3, identifying it as a critical molecular switch that couples tumor aggressiveness with microenvironmental remodeling, thus presenting a promising therapeutic target for HCC.

Keywords

Hepatocellular carcinoma; Macrophage polarization; PTGES3; SP1; TGF-β; Tumor microenvironment.

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