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MES  (Synonyms: 2-Morpholinoethanesulphonic acid)

製品番号: HY-D0858 純度: 99.85%
COA 取扱説明書 Technical Support

MES (2-Morpholinoethanesulphonic acid) is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies.

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MES

MES 構造式

CAS 番号 : 4432-31-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
500 mg $25 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 4 publication(s) in Google Scholar

Other Forms of MES:

MES 関連抗体

Top Publications Citing Use of Products

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  • 純度とドキュメンテーション

  • 参考文献

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製品説明

MES (2-Morpholinoethanesulphonic acid) is a kind of amphoteric ion buffer, the buffer capacity ranging pH 5.5-7.0. As a Good's buffer, MES is widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis and protein studies[1][2].

体外実験

MES is a zwitterionic buffer and can provide effective buffering capacity at around pH 6.15. The pKa value of MES changes with temperature, so the change of its pKa value needs to be considered when using it at different temperatures[1].
MES shows good buffering properties in biological experiments, exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), thereby can be used in studies involving metal ions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MES 関連抗体
分子量

195.24

分子式

C6H13NO4S
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCN1CCOCC1)(O)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外: 

H2O : 125 mg/mL (640.24 mM; Need ultrasonic)

DMSO : 50 mg/mL (256.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.1219 mL 25.6095 mL 51.2190 mL
5 mM 1.0244 mL 5.1219 mL 10.2438 mL
10 mM 0.5122 mL 2.5610 mL 5.1219 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (256.10 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.93%

COA (247 KB) HNMR (261 KB) RP-HPLC (202 KB) MS (69 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 5.1219 mL 25.6095 mL 51.2190 mL 128.0475 mL
5 mM 1.0244 mL 5.1219 mL 10.2438 mL 25.6095 mL
10 mM 0.5122 mL 2.5610 mL 5.1219 mL 12.8048 mL
15 mM 0.3415 mL 1.7073 mL 3.4146 mL 8.5365 mL
20 mM 0.2561 mL 1.2805 mL 2.5610 mL 6.4024 mL
25 mM 0.2049 mL 1.0244 mL 2.0488 mL 5.1219 mL
30 mM 0.1707 mL 0.8537 mL 1.7073 mL 4.2683 mL
40 mM 0.1280 mL 0.6402 mL 1.2805 mL 3.2012 mL
50 mM 0.1024 mL 0.5122 mL 1.0244 mL 2.5610 mL
60 mM 0.0854 mL 0.4268 mL 0.8537 mL 2.1341 mL
80 mM 0.0640 mL 0.3201 mL 0.6402 mL 1.6006 mL
100 mM 0.0512 mL 0.2561 mL 0.5122 mL 1.2805 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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MES Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MES4432-31-92-Morpholinoethanesulphonic acidBiochemical Assay ReagentsGood's buffersplants culture mediumreagent solutionphysiological experimentsInhibitorinhibitorinhibit

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