MNI-caged-L-glutamate 

MNI-caged-L-glutamate is an inert photolabile glutamate precursor. MNI-caged-L-glutamate does not modulate NMDA and AMPA receptor function in cultured neurones. MNI-caged-L-glutamate can be used for research on neurotransmitters^[1].

MNI-caged L-glutamate (0.1 mM; single 1 ms flashes, repeated up to 8 times) undergoes photolytic conversion with 30-38% efficiency per 1 ms flash in aqueous vesicles^[1].
MNI-caged L-glutamate (250-1000 μM; 5-10 min) does not act as an antagonist at AMPARs in cultured rat hippocampal neurones, and photoreleased L-glutamate activates AMPAR-mediated currents with a half-rise time of 0.78 ms^[1].
MNI-caged L-glutamate (500 μM; 5-10 min) does not act as an antagonist at NMDARs in cultured rat hippocampal neurones^[1].
MNI-caged L-glutamate (1 mM; 5-10 min) does not alter GABA_A R-mediated spontaneous current amplitudes in cultured rat hippocampal neurones^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

323.30

C₁₄H₁₇N₃O₆

295325-62-1

Solid

Light yellow to yellow

OC([C@@H](N)CCC(N1C2=C([N+]([O-])=O)C=CC(OC)=C2CC1)=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

• [1]. Canepari M, et al. Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters. J Neurosci Methods. 2001;112(1):29-42.  [Content Brief]