N-Hydroxysuccinimide (Synonyms: HOSu; 1-Hydroxy-2,5-pyrrolidinedione)

Name: N-Hydroxysuccinimide
Brand: MedChemExpress
SKU: HY-Y0623
Rating: 5.0 (8 reviews)

Cat. No.: HY-Y0623 Pureza: 99.98%: Ficha de datos
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N-Hydroxysuccinimide (HOSu; 1-Hydroxy-2,5-pyrrolidinedione) is a covalent crosslinker commonly used in bioconjugation technology with a primary amine group. N-Hydroxysuccinimide reacts with amino groups (-NH2) to form a stable amide bond, which can modify amino-containing biomolecules. N-Hydroxysuccinimide can be used, for example, for protein labeling with fluorescent dyes and enzymes, surface activation of chromatography supports, microbeads, nanoparticles and microarray slides, and chemical synthesis of peptides. N-Hydroxysuccinimide has a wide range of applications in biomaterial synthesis (such as collagen, chitosan crosslinking), drug delivery systems (such as hydrogel preparation) and tissue engineering.

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N-Hydroxysuccinimide Estructura química

No. CAS : 6066-82-6

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 g	En stock	Estimated Time of Arrival: December 31
10 g	En stock	Estimated Time of Arrival: December 31
50 g	En stock	Estimated Time of Arrival: December 31
100 g	En stock	Estimated Time of Arrival: December 31
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Revisión del cliente

Based on 8 publication(s) in Google Scholar

8 Publications Citing Use of MCE N-Hydroxysuccinimide

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•Related Proteins:

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Descripciòn

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	> 50 μM Compound: NHS	Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins by cell titer-glo assay Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins by cell titer-glo assay	[PMID: 35859867]
HEK293	IC₅₀	> 50 μM Compound: NHS	Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay	[PMID: 35859867]
HEK293	IC₅₀	> 50 μM Compound: NHS	Cytotoxicity against permeabilized HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay Cytotoxicity against permeabilized HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay	[PMID: 35859867]

In Vitro

In the cross-linking experiment of collagen solution, N-Hydroxysuccinimide-activated adipic acid was used to confirm the formation of intermolecular cross-linking by SDS-PAGE. Fluorescence spectroscopy showed that the polarity of the collagen microenvironment increased after cross-linking. Atomic force microscopy observed that collagen molecules randomly aggregated, which can improve the self-assembly ability of collagen^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

115.09

Fòrmula

C₄H₅NO₃

No. CAS

6066-82-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1O)CCC1=O

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

H₂O : ≥ 100 mg/mL (868.89 mM)

DMSO : 100 mg/mL (868.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.6889 mL	43.4443 mL	86.8885 mL
5 mM	1.7378 mL	8.6889 mL	17.3777 mL
10 mM	0.8689 mL	4.3444 mL	8.6889 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.99%

Select Batch:

Ficha de datos (276 KB) SDS (416 KB)

COA (314 KB) HNMR (126 KB) RP-HPLC (226 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Zhang M, et al. Interactions of collagen molecules in the presence of N-hydroxysuccinimide activated adipic acid (NHS-AA) as a crosslinking agent. Int J Biol Macromol. 2011 Nov 1;49(4):847-54. [Content Brief]

[2]. Ishikawa S, et al. N-Hydroxysuccinimide Bifunctionalized Triblock Cross-Linker Having Hydrolysis Property for a Biodegradable and Injectable Hydrogel System. ACS Biomater Sci Eng. 2019 Nov 11;5(11):5759-5769. [Content Brief]

[3]. Klykov O, et al. Quantification of N-hydroxysuccinimide and N-hydroxysulfosuccinimide by hydrophilic interaction chromatography (HILIC)[J]. Analytical methods, 2015, 7(15): 6443-6448.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.6889 mL	43.4443 mL	86.8885 mL	217.2213 mL
	5 mM	1.7378 mL	8.6889 mL	17.3777 mL	43.4443 mL
	10 mM	0.8689 mL	4.3444 mL	8.6889 mL	21.7221 mL
	15 mM	0.5793 mL	2.8963 mL	5.7926 mL	14.4814 mL
	20 mM	0.4344 mL	2.1722 mL	4.3444 mL	10.8611 mL
	25 mM	0.3476 mL	1.7378 mL	3.4755 mL	8.6889 mL
	30 mM	0.2896 mL	1.4481 mL	2.8963 mL	7.2407 mL
	40 mM	0.2172 mL	1.0861 mL	2.1722 mL	5.4305 mL
	50 mM	0.1738 mL	0.8689 mL	1.7378 mL	4.3444 mL
	60 mM	0.1448 mL	0.7241 mL	1.4481 mL	3.6204 mL
	80 mM	0.1086 mL	0.5431 mL	1.0861 mL	2.7153 mL
	100 mM	0.0869 mL	0.4344 mL	0.8689 mL	2.1722 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

N-Hydroxysuccinimide Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Hydroxysuccinimide6066-82-6HOSu 1-Hydroxy-2,5-pyrrolidinedioneBiochemical Assay ReagentsInhibitorinhibitorinhibit

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N-Hydroxysuccinimide (Synonyms: HOSu; 1-Hydroxy-2,5-pyrrolidinedione)

Revisión del cliente

N-Hydroxysuccinimide Related Antibodies

8 Publications Citing Use of MCE N-Hydroxysuccinimide

Recomendaciones destacadas

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

N-Hydroxysuccinimide Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

N-Hydroxysuccinimide Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report