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  4. NDSB 256-4T

NDSB 256-4T  (Synonyms: 3-(4-(tert-Butyl)pyridinio)-1-propanesulfonate)

Cat. No.: HY-W127622 Pureté: 98.0%
COA
SDS
Instruction de manipulation Technical Support

NDSB 256-4T is a non-washing sulfabetaine compound. NDSB-256-4T prevents protein aggregation and promotes protein folding by interacting with early folding intermediates.

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NDSB 256-4T

NDSB 256-4T Chemical Structure

CAS No. : 570412-84-9

Size Prix Stock Quantité
Échantillon gratuit (0.1 - 0.2 mg)   Appliquer maintenant  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
1 g En stock
5 g   Obtenir un devis  
10 g   Obtenir un devis  

* Veuillez sélectionner la quantité avant d'ajouter des articles.

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NDSB 256-4T Related Antibodies

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  • Activité biologique

  • Pureté et documentation

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Description

NDSB 256-4T is a non-washing sulfabetaine compound. NDSB-256-4T prevents protein aggregation and promotes protein folding by interacting with early folding intermediates[1].

Masse moléculaire

257.35

Formule

C12H19NO3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C)(C)C1=CC=[N+](CCCS(=O)(=O)[O-])C=C1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

DMSO : 50 mg/mL (194.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8858 mL 19.4288 mL 38.8576 mL
5 mM 0.7772 mL 3.8858 mL 7.7715 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (19.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (19.43 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: 99.62%

SDS (393 KB)

COA (246 KB) LCMS (288 KB)

Instruction de manipulation (2659 KB)
Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8858 mL 19.4288 mL 38.8576 mL 97.1440 mL
5 mM 0.7772 mL 3.8858 mL 7.7715 mL 19.4288 mL
10 mM 0.3886 mL 1.9429 mL 3.8858 mL 9.7144 mL
15 mM 0.2591 mL 1.2953 mL 2.5905 mL 6.4763 mL
20 mM 0.1943 mL 0.9714 mL 1.9429 mL 4.8572 mL
25 mM 0.1554 mL 0.7772 mL 1.5543 mL 3.8858 mL
30 mM 0.1295 mL 0.6476 mL 1.2953 mL 3.2381 mL
40 mM 0.0971 mL 0.4857 mL 0.9714 mL 2.4286 mL
50 mM 0.0777 mL 0.3886 mL 0.7772 mL 1.9429 mL
60 mM 0.0648 mL 0.3238 mL 0.6476 mL 1.6191 mL
80 mM 0.0486 mL 0.2429 mL 0.4857 mL 1.2143 mL
100 mM 0.0389 mL 0.1943 mL 0.3886 mL 0.9714 mL
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NDSB 256-4T Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NDSB 256-4T570412-84-93-(4-(tert-Butyl)pyridinio)-1-propanesulfonateBiochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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