Nuclease P1  (Synonyms: ヌクレアーゼP1)

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries^[1]. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.

Reaction condition
pH range: Effective pH range: 4.0-8.0, optimal pH range: 4.5-5.5
Temperature range: Effective temperature range: 35°C-85°C, optimal temperature range: 65°C-75°C

Protocol Yeast nucleic acid (RNA) is used as the substrate, the substrate concentration is 2%-5%, the recommended enzyme addition amount is 2%-4%(based on the dry matter of the substrate), and the enzymatic hydrolysis time under the optimal conditions is 7 h-9 h;
Yeast is used as the substrate, the yeast concentration is 10%-12%, the nuclease amount is 0.3%-0.5%(based on dry yeast matter), and the enzymatic hydrolysis time under the optimal conditions is 7 h-9 h.
Note: Due to differences in application fields and experimental parameters, the actual addition method and amount of this product should be determined through testing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

54576-84-0

Solid

White to light yellow

[Nuclease P1]

3.1.30.1

≥200 U/mg protein

3' 5”-Phosphodiesterase: One unit is defined as the amount of enzyme that releas 1 μmol acidsoluble nucleotides from RNA at 37°C and pH 5.3, in per minute；3`-Nuclease: One unit is defined as the amount of enzyme that hydrolyze 1 μmol of orthophosphoric acid from 3`-AMP in per minute, at 37°C and pH 7.2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mitiushkina NV, et al. Preparation of Duplex Sequencing Libraries for Archival Paraffin-Embedded Tissue Samples Using Single-Strand-Specific Nuclease P1. Int J Mol Sci. 2022 Apr 21;23(9):4586.  [Content Brief]