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  4. Peroxyfluor 1

Peroxyfluor 1 is a cell-permeable probe for H2O2. Peroxyfluor 1 represents a first-generation, green-fluorescent probe.

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Peroxyfluor 1

Peroxyfluor 1 構造式

CAS 番号 : 817170-66-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 554 在庫あり
Solution
10 mM * 1 mL in DMSO USD 554 在庫あり
Solid
5 mg $456 在庫あり
10 mg $729 在庫あり
25 mg $1530 在庫あり
50 mg $2440 在庫あり
100 mg $3900 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Peroxyfluor 1 関連抗体

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  • 生物活性

  • 純度とドキュメンテーション

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製品説明

Peroxyfluor 1 is a cell-permeable probe for H2O2. Peroxyfluor 1 represents a first-generation, green-fluorescent probe[1].

体外実験

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[1]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 5 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 5 µM with PBS or cell culture medium.
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Peroxyfluor 1 (5 μM) is incubated with live cell samples for 5-10 min.
2.2 Addition of H2O2 (10-100 μM) to cell samples for 15-30 min.
2.3 Fluorescence microscopy detection (Ex/Em = 450/530 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peroxyfluor 1 関連抗体
分子量

552.23

分子式

C32H34B2O7
Emission (Em)

530
Excitation (Ex)

450
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C1OC2(C3=C1C=CC=C3)C4=CC=C(B5OC(C)(C(C)(C)O5)C)C=C4OC6=CC(B7OC(C)(C(C)(C)O7)C)=CC=C62
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 14.29 mg/mL (25.88 mM; ultrasonic and warming and adjust pH to 10 with 1M NaOH; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8108 mL 9.0542 mL 18.1084 mL
5 mM 0.3622 mL 1.8108 mL 3.6217 mL
10 mM 0.1811 mL 0.9054 mL 1.8108 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8108 mL 9.0542 mL 18.1084 mL 45.2710 mL
5 mM 0.3622 mL 1.8108 mL 3.6217 mL 9.0542 mL
10 mM 0.1811 mL 0.9054 mL 1.8108 mL 4.5271 mL
15 mM 0.1207 mL 0.6036 mL 1.2072 mL 3.0181 mL
20 mM 0.0905 mL 0.4527 mL 0.9054 mL 2.2635 mL
25 mM 0.0724 mL 0.3622 mL 0.7243 mL 1.8108 mL
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Peroxyfluor 1 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Peroxyfluor 1817170-66-4Peroxyfluor1Peroxyfluor-1Fluorescent Dyecell-permeable probeH2O2green-fluorescent probeInhibitorinhibitorinhibit

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製品名:
Peroxyfluor 1
製品番号:
HY-D1458
数量:
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