Propidium monoazide 

Propidium monoazide is a cell-impermeant photoreactive DNA-binding dye that preferentially binds to dsDNA. Propidium monoazide (PMA) prevents DNA from dead bacteria from being amplified during the PCR. PMA-PCR enhanced both the specificity and the sensitivity of PCR^[1]. Propidium monoazide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
Propidium monoazide-PCR^[1]
Propidium monoazide is used to label bacteria. In a dimly lit room, add 5 μL of 2 mM photoreaction dye Propidium monoazide to 500 mL of the sample, resulting in a final concentration of 20 μM/μL.
2) Incubate the sample in the dark for 5 minutes (cover with aluminum foil), and gently shake the tube to ensure thorough mixing. Perform light activation with the PMA-Lite^TM LED light source for 10 minutes.
2. Extract bacterial DNA 3. Perform PCR.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

511.49

C₂₇H₃₂Cl₂N₆

91416-20-5

Solid

Brown to reddish brown

[N-]=[N+]=NC1=CC2=C(C3=C([N+](CCC[N+](CC)(CC)C)=C2C4=CC=CC=C4)C=C(N)C=C3)C=C1.[Cl-].[Cl-]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Mohamed Askar 1, et al. Propidium monoazide-polymerase chain reaction for detection of residual periprosthetic joint infection in two-stage revision. Mol Biol Rep. 2019 Dec;46(6):6463-6470.  [Content Brief]