2. Recombinant Proteins
  3. Enzymes & Regulators Biotinylated Proteins
  4. Hydrolases (EC 3) Phosphatase
  5. Alkaline Phosphatase
  6. Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi)

Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P700951
Handling Instructions Technical Support

The Alkaline Phosphatase/ALPG protein, an enzyme, hydrolyzes phosphate compounds, breaking down various molecules. It acts as an alkaline phosphatase, cleaving phosphate esters and rendering molecules inactive. This versatile enzyme is vital in processes like bone mineralization, cellular signaling, and metabolism. Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

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제품 설명

The Alkaline Phosphatase/ALPG protein, an enzyme, hydrolyzes phosphate compounds, breaking down various molecules. It acts as an alkaline phosphatase, cleaving phosphate esters and rendering molecules inactive. This versatile enzyme is vital in processes like bone mineralization, cellular signaling, and metabolism. Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background

The Alkaline Phosphatase/ALPG protein is an enzyme that possesses the capability to hydrolyze a range of phosphate compounds. It exhibits the ability to break down various phosphate molecules through its enzymatic activity. This protein acts as an alkaline phosphatase, catalyzing the hydrolysis of phosphate esters in an alkaline environment. Its enzymatic activity allows it to cleave phosphate groups from molecules such as nucleotides, proteins, and phospholipids, rendering them inactive. This versatile enzyme plays a vital role in various physiological processes, including bone mineralization, cellular signaling, and metabolism.
Species

Human
Source

HEK293
Tag

C-Avi;C-8*His
Accession

P10696 (I20-D503)
Gene ID

251  [NCBI]
Molecular Construction
N-term
ALPG (I20-D503)
Accession # P10696
8*His-Avi
C-term
Protein Length

Full Length of Mature Protein
Conjugation

Biotin
Synonyms
Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2
AA Sequence

IIPVEEENPDFWNRQAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTD

Predicted Molecular Mass
55.5 kDa
분자량

Approximately 56-70 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Glycosylation
Yes
Purity

≥ 95%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
각종 서류
Handling Instructions (2659 KB)

Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
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