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  5. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST)

AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST)

Cat. No.: HY-P76144
Handling Instructions

The AMPK gamma 1 is the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), a crucial regulator of cellular energy metabolism. Activated by low ATP levels, it enhances energy production while suppressing biosynthetic processes and cell growth. The gamma subunit mediates AMP, ADP, and ATP binding, influencing AMPK activation or inhibition. Allosteric AMP binding activates the alpha subunit, while ADP stimulates phosphorylation, and ATP promotes dephosphorylation, rendering AMPK inactive. Additionally, AMPK modulates cellular polarity and interacts with FNIP1 and FNIP2. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST) is the recombinant human-derived AMPK gamma 1/beta 2/alpha 2 Heterotrimer protein, expressed by Sf9 insect cells, with N-His, N-GST labeled tag. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST), has molecular weight of ~35 & 37 & 95 KDa, respectively.

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Description

The AMPK gamma 1 is the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), a crucial regulator of cellular energy metabolism. Activated by low ATP levels, it enhances energy production while suppressing biosynthetic processes and cell growth. The gamma subunit mediates AMP, ADP, and ATP binding, influencing AMPK activation or inhibition. Allosteric AMP binding activates the alpha subunit, while ADP stimulates phosphorylation, and ATP promotes dephosphorylation, rendering AMPK inactive. Additionally, AMPK modulates cellular polarity and interacts with FNIP1 and FNIP2. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST) is the recombinant human-derived AMPK gamma 1/beta 2/alpha 2 Heterotrimer protein, expressed by Sf9 insect cells, with N-His, N-GST labeled tag. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST), has molecular weight of ~35 & 37 & 95 KDa, respectively.

Background

The AMPK gamma 1 serves as the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), a pivotal regulator of cellular energy metabolism. When intracellular ATP levels decrease, AMPK is activated, initiating a cascade of events that enhance energy production while suppressing energy-consuming processes such as protein, carbohydrate, and lipid biosynthesis, as well as cell growth and proliferation. The gamma non-catalytic subunit of AMPK mediates binding to AMP, ADP, and ATP, influencing the activation or inhibition of AMPK. AMP binding allosterically activates the alpha catalytic subunit by inducing phosphorylation and preventing dephosphorylation. ADP stimulates phosphorylation, while ATP promotes dephosphorylation, rendering the AMPK enzyme inactive. Beyond its role in energy regulation, AMPK acts as a modulator of cellular polarity by remodeling the actin cytoskeleton, potentially through the indirect activation of myosin. The heterotrimeric composition of AMPK including an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta subunit (PRKAB1 or PRKAB2), and a gamma non-catalytic subunit (PRKAG1, PRKAG2, or PRKAG3), highlighting its intricate regulatory mechanisms. AMPK further interacts with FNIP1 and FNIP2 to modulate its cellular functions.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

P54619 (M1-P331)&O43741 (M1-I272)&P54646 (M1-R552)

Gene ID

5571  [NCBI]&5565  [NCBI]&5563  [NCBI]

Molecular Construction
N-term
PRKAG1 (M1-P331)
Accession # P54619
C-term
N-term
PRKAB2 (M1-I272)
Accession # O43741
C-term
N-term
His-GST
PRKAA2 (M1-R552)
Accession # P54646
C-term
Synonyms
5'-AMP-activated protein kinase; AMPK; AMPK (α2, β2, γ1) Protein
Molecular Weight

Approximately 35&37&95 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST)
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HY-P76144
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