AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (Active, sf9, His-GST)

AMPK gamma 1 is the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK) and is an important regulator of cellular energy metabolism. It is activated by low ATP levels, enhancing energy production while inhibiting biosynthetic processes and cell growth. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST) is the recombinant human-derived AMPK gamma 1/beta 2/alpha 2 Heterotrimer protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (sf9, His-GST), has molecular weight of ~35 & 37 & 95 KDa, respectively.

The AMPK gamma 1/beta 1/alpha 1 heterotrimer serves as the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), a pivotal regulator of cellular energy metabolism. When intracellular ATP levels decrease, AMPK is activated, initiating a cascade of events that enhance energy production while suppressing energy-consuming processes such as protein, carbohydrate, and lipid biosynthesis, as well as cell growth and proliferation. The gamma non-catalytic subunit of AMPK mediates binding to AMP, ADP, and ATP, influencing the activation or inhibition of AMPK. AMP binding allosterically activates the alpha catalytic subunit by inducing phosphorylation and preventing dephosphorylation. ADP stimulates phosphorylation, while ATP promotes dephosphorylation, rendering the AMPK enzyme inactive. Beyond its role in energy regulation, AMPK acts as a modulator of cellular polarity by remodeling the actin cytoskeleton, potentially through the indirect activation of myosin. The heterotrimeric composition of AMPK includes an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta subunit (PRKAB1 or PRKAB2), and a gamma non-catalytic subunit (PRKAG1, PRKAG2, or PRKAG3), highlighting its intricate regulatory mechanisms. AMPK further interacts with FNIP1 and FNIP2 to modulate its cellular functions.

The specific activity was determined to be 1-6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.

Human

Sf9 insect cells

N-10*His;N-His;N-GST

P54619-1 (M1-P331)&O43741 (M1-I272)&P54646 (M1-R552)

5571  [NCBI]&5565  [NCBI]&5563  [NCBI]

Approximately 35&37&95 kDa.

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10% glycerol, 1 mM GSH, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.