Angiopoietin-4 Protein, Human (HEK293, Fc)

Angiopoietin-4 Protein (ANGPT4), crucially binding to TEK/TIE2, modulates ANGPT1 signaling and induces TEK/TIE2 phosphorylation. Beyond regulation, ANGPT4 promotes endothelial cell survival, migration, and angiogenesis. Structurally, it forms a disulfide-linked homodimer, highlighting its oligomeric nature. Functional interactions with TEK/TIE2 underscore ANGPT4's integral role in orchestrating essential cellular processes for angiogenesis and vascular homeostasis. Angiopoietin-4 Protein, Human (HEK293, Fc) is the recombinant human-derived Angiopoietin-4 protein, expressed by HEK293, with N-hFc labeled tag. The total length of Angiopoietin-4 Protein, Human (HEK293, Fc) is 222 a.a., with molecular weight of ~66 kDa.

The Angiopoietin-4 (ANGPT4) protein exhibits a pivotal role as it binds to TEK/TIE2, thereby modulating ANGPT1 signaling. Beyond its regulatory function, ANGPT4 has the capability to induce tyrosine phosphorylation of TEK/TIE2. Notably, it plays a crucial role in promoting endothelial cell survival, migration, and angiogenesis. Structurally, ANGPT4 exists as a homodimer, connected by disulfide linkages, highlighting its oligomeric nature. Its functional interactions with TEK/TIE2 further emphasize its integral role in orchestrating cellular processes essential for angiogenesis and vascular homeostasis.

Angiopoietin-4 acts as an angiogenic inhibitor, and it (50 ng/mL; 8 h, 24 h) reduces endothelial migration and tube formation in co-cultured HUVECs with transfected fibroblasts^[1].

Human

HEK293

N-hFc

Q9Y264 (M282-I503)

51378  [NCBI]

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Dittrich GM, et al. Fibroblast GATA-4 and GATA-6 promote myocardial adaptation to pressure overload by enhancing cardiac angiogenesis. Basic Res Cardiol. 2021 Apr 19;116(1):26.  [Content Brief]

  [2]. Wu Z, et al. Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration. Protein Cell. 2023 May 8;14(5):350-368.  [Content Brief]